Creative BioMart is a well-known expert specializing in the application of advanced cell surface display technology to accelerate the release of protein engineering. With years of experience, we are able to provide yeast surface display system service based on cell surface technology to precisely meet customer requirements.

Yeast Surface Display System is a eukaryotic protein expression system. The gene encoding the target protein with the secretion signal peptide is fused with the gene encoding ankyrin, and then the fusion gene is introduced into yeast cells and expressed under the control of any promoter. At present, the yeast surface display system combined with fluorescence activated cell sorting (FACS) technology and directed evolution has established a mature high-throughput screening method for protein engineering, which has a wide range of applications in many industrial fields such as medicine, food, and biofuels.

Saccharomyces cerevisiae Surface Display System Services

We provide a yeast surface display platform services base on the lectin display system and flocculin display system. We use Saccharomyces cerevisiae as a host cell, and provide a variety of options for cell surface anchoring proteins, including Agα1p, Aga2p, Cwp1p, Cwp2p, Tip1p, Flo1p, Sed1p, YCR89w, Tir1, etc. You can choose a suitable yeast surface display system according to your needs.

The Saccharomyces cerevisiae surface display platform we provide can be applied to protein epitope mapping, protein-protein interaction identification, biotechnology, biomedical applications and other fields.

Fig 1. Schematic representation of yeast surface display. (Cherf GM, et al., 2015)

Features of Saccharomyces cerevisiae Surface Display System

• Fast and quantitative library screening through fluorescence activated cell sorting.
• Minimize artifacts caused by host expression bias through concurrent expression marking.
• The mutation characteristics (such as affinity, stability) can be easily evaluated and displayed in the form of surface display, without the need for soluble expression and purification of each individual clone.
• Eukaryotic expression system can integrate post-translational modifications, such as disulfide bond formation.
• Compared with other eukaryotic display systems, the Saccharomyces cerevisiae display system has low technical and time requirements.

Creative BioMart has been continuously developing cutting-edge cell surface display technology, and is committed to providing a flexible and efficient customized yeast surface display system service. Based on the protein engineering platform, we have successfully constructed an impeccable cell surface display library of various types. We are proud to be able to screen and select proteins with the required characteristics from a large number of variants to best accelerate the research of life sciences. If you are interested in our services, please do not hesitate to contact us for more information.

Reference

1. Cherf GM, Cochran JR. (2015) Applications of Yeast Surface Display for Protein Engineering. Methods Mol Biol. 1319:155-75.

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