After decades of advances in computational protein design, protein design that folds and functions in lipid membranes is now a frontier field. Membrane proteins are designed and redesigned to better understand and engineer membrane protein structure and function. With the support of our advanced protein engineering platform established for many years, Creative BioMart provides a comprehensive customized knowledge-based membrane protein design service for designing functional membrane proteins and generating transmembrane protein scaffolds.

Membrane proteins are involved in a variety of cellular processes, including selective molecular transport across bilayers, uptake of nutrients, emission of toxins and waste, respiration, cell motility, and cell signaling. These proteins constitute most of the known drug targets. Therefore, more work is required to obtain detailed predictive knowledge of the molecular basis of its structural stability, and activities. While many membrane proteins are considered high-priority targets for drug design, the inherent insolubility and instability of the transmembrane domain hinders obtaining high-resolution crystals to specifically study structure-function relationships. Directed evolution and computational design have great potential as tools for studying membrane proteins. Directed evolution has been successfully applied to membrane proteins in several ways, including the evolution of better expressed receptors, more versatile co-crystallizing agents, and transmembrane targeting peptides. Design specific inhibitors of transmembrane proteins or redesign membrane proteins for better structurally defined expression through rational design strategies.

Fig 1. Functional membrane protein designs. (Vorobieva AA, 2021)

Services

Membrane protein systems in nature are often very complex, so it is desirable to create systems in which the details of function and structure are more easily controlled by researchers. Designing and redesigning membrane proteins allows for better understanding and engineering of membrane protein structure and function. Based on a rational design-assisted directed evolution strategy, Creative BioMart has successfully established an engineered membrane protein platform for exploring membrane protein structures, introducing new functions, regulating membrane integrity, controlling the activities of receptors and integrins, and obtaining water-soluble variants of membrane proteins. body. Our aim is to use empirical knowledge of structure, sequence and native proteins to create new membrane proteins.

Rational computational design is an efficient and economical approach for engineering membrane proteins. We provide the most specialized solutions for the low protein expression and instability encountered in membrane protein design, the extreme difficulty of obtaining atomic resolution structural data, and the regulation of transmembrane domains. We provide one-stop service for engineered membrane proteins:

• Identify and quantify membrane protein-specific features and structures.
• Modeling and computational analysis of membrane protein structures.
• Structural determination and functional characterization of membrane proteins.

Our knowledge-based membrane protein design service makes the study of membrane proteins more informative and effective. We engineer membrane proteins to be conduits for the transmembrane flow of ions, electrons, small molecules, and (in the case of signaling) information. In addition, it can be used to modulate the functional properties of membrane-associated proteins, such as transmembrane helical peptides targeting specific integrin transmembrane domains.

Service Principle

Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.

We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. If you are interested in our services, please do not hesitate to contact us for more information or to discuss in detail.

References

1. Vorobieva AA. (2021) Principles and Methods in Computational Membrane Protein Design. J Mol Biol. 433(20):167154.
2. Perez-Aguilar J M, Saven J G. (2012) Computational design of membrane proteins[J]. Structure. 20(1): 5-14.

• Knowledge-Based Novel Protein Folds Design
• Knowledge-Based Preorganized Structure Design
• Knowledge-Based Secondary Structure Self-Assembly Design
• Knowledge-Based Single-Chain Protein Design