Creative BioMart is a well-known expert specializing in the application of advanced cell surface display technology to accelerate the release of protein engineering. With years of experience, we are able to provide baculovirus-insect cell display system service based on cell surface technology to precisely meet customer requirements.

Baculovirus-insect cell display system is a higher eukaryotic display system. The display system uses baculovirus as a vector, the foreign gene is inserted into the capsid protein gene or envelope protein gene of the virus for fusion expression, and displayed on the surface of virus particles or infected cells. With its simple process, high expression level and post-translational modification function, it can prepare large-scale post-translationally modified proteins in a short time, which is widely used in virus vaccine development, signal protein preparation, cytokine preparation, protein library screening, etc.

Baculovirus-Insect Cell Display System Services

We provide a baculovirus-insect cell display platform mainly using AcNPV (Spodoptera californica nuclear polyhedrosis virus)-sf9 (Spodoptera frugiperda cell line) cells and BmNPV (Bombyx mori nuclear polyhedrosis virus)-BmN cells Expression system. Multiple expression systems, multiple hosts, and multiple vectors are available for your choice.

The genome of baculovirus is a single closed circular double-stranded DNA molecule with a size of 80-160 kb. Its genome can be replicated and transcribed in the insect cell nucleus. The baculovirus-insect cell display system we provided allows the insertion of large fragments of foreign genes. The foreign protein can be effectively displayed on the surface of the virus particle, and the foreign protein can be subjected to post-translational modifications such as glycosylation processing and folding.

The baculovirus-insect cell display display service we provide is especially suitable for display of higher eukaryotic cell surface proteins and secreted proteins that require specific post-translational processing to effectively fold and have biological activity.

Fig 1. Baculovirus as Mammalian Cell Expression Vector for Gene Therapy: An Emerging Strategy. (Cherf GM, et al., 2015)

Features of Baculovirus-Insect Cell Display System

• The attractiveness of this system lies in the combination of simple cloning technology and virus propagation with the eukaryotic post-translational modification mechanism of insect cells.
• This eukaryotic display system can display large and complex proteins on the surface of baculovirus particles, improving the efficiency and specificity of virus binding and entering mammalian cells.
• Two display systems, AcNPV and BmNPV, are used to quickly construct recombinant baculovirus plasmids with simple bacterial mixing, with high positive rate and short cycle.
• The system has the ability to process eukaryotic proteins, including the ability to modify proteins such as phosphorylation or glycosylation.

Creative BioMart has been continuously developing cutting-edge cell surface display technology, and is committed to providing a flexible and efficient customized baculovirus-insect cell display system service. Based on the protein engineering platform, we have successfully constructed an impeccable cell surface display library of various types. We are proud to be able to screen and select proteins with the required characteristics from a large number of variants to best accelerate the research of life sciences. If you are interested in our services, please do not hesitate to contact us for more information.

References

1. Ghosh S, Parvez MK, Banerjee K, et al.. (2002) Baculovirus as mammalian cell expression vector for gene therapy: an emerging strategy. Mol Ther. 6(1): 5-11.
2. Oker-Blom C, Airenne KJ, Grabherr R. (2003) Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery. Brief Funct Genomic Proteomic. 2(3):244-53.

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