Creative BioMart is a well-known expert specializing in the application of advanced cell surface display technology to accelerate the release of protein engineering. With years of experience, we are able to provide bacteria surface display system service based on cell surface technology to precisely meet customer requirements.

Bacteria surface display technology combines a fluorescence activated cell screener or a flow cytometer screener for high-throughput screening, expressing heterologous proteins on the surface of Gram-positive and Gram-negative bacterial cells. Bacteria surface display links the function of a protein with the gene encoding it, and can be used to find target proteins with desired characteristics and prepare affinity ligands. Bacterial surface display can be used for a variety of applications, including affinity-based screening, antibody epitope mapping, identification of peptide substrates, identification of cell-bound peptides, and vaccine production.

Bacteria Surface Display System Services

We provide a variety of bacterial display scaffolds for library screening according to the size of the carrier protein: OmpA, OprF, LamB, PAL, TraT, SLH, PA, M6, DppE, etc. E. coli bacteria and staphylococcus bacteria as host bacteria are the most widely used bacteria surface display system. Each display system has its own advantages and disadvantages, you can choose a suitable bacteria surface display system according to your needs.

The bacterial surface display platform we provide is widely used in vaccine development, protein library and peptide library screening, whole-cell catalysts, whole-cell adsorbents and degradants, etc.

Fig 1. Different types of carrier proteins found on the surface of the Gram-positive bacteria. (Shabnam Parwin, et al., 2019)

Features of Bacteria Surface Display System

• Ability to determine the functional characteristics of each clone in the mutant library.
• At present, the only method that can quantitatively test the single cell level and the catalytic activity of a large number of mutants.
• Quantitative analysis of enzyme activity.
• Simultaneously detect multiple parameters and record each observed clone.
• Compared with bacteriophages, bacteria surface display technology has unique advantages in vaccine applications, allowing faster and more efficient screening.

Creative BioMart has been continuously developing cutting-edge cell surface display technology, and is committed to providing a flexible and efficient customized bacteria surface display system service. Based on the protein engineering platform, we have successfully constructed an impeccable cell surface display library of various types. We are proud to be able to screen and select proteins with the required characteristics from a large number of variants to best accelerate the research of life sciences. If you are interested in our services, please do not hesitate to contact us for more information.

Reference

1. Shabnam Parwin, Sashi Kalan, Preeti Srivastava. (2019) Bacterial Cell Surface Display. ACS. Doi: 10.1021/bk-2019- 1329.ch005.

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