New technologies to generate transgenic plants in herbaceous crops and even trees, the increase in the number of plant whole genome sequences, and the chemical diversity of plants provide enormous sources of protein engineering. Based on our extensive experience in protein engineering, researchers apply it to optimize desired traits in plants. Creative BioMart is committed to introducing genes for proteins of a given trait of any given species into crops of interest, thereby conferring a desired trait or phenotype. We have the ability to provide global customers with the best solutions for protein engineering applications in agriculture.

Background

To sustain life, food must provide adequate calories and nutrients. Food insecurity, the lack of access to adequate food supplies, threatens malnutrition for millions of people around the world. And with a rapidly growing world population, competition for space and climate change, ensuring food security is a global concern. Traditional plant breeding is limited to acquiring genes from closely related species and is not fast enough to improve crop characteristics. Genome sequencing technologies have produced multiple genes with many different functions from many different species, and what scientists need to do is introduce any gene for a protein with the desired function into the crop of interest. Due to compromises in expression, folding, and stability, this protein must be engineered or redesigned to achieve the ultimate goal. Protein engineering of crop species offers promising solutions for agricultural development. Studies show that scientists have successfully used protein engineering to identify new glyphosate-resistant ESP synthase mutants.

Fig 1. Library screening program in plant research. (Engqvist M K M, et al., 2019)

Custom Solutions for Agriculture

Protein engineering has great potential in improving plants or improving and generating metabolic pathways of interest, allowing the introduction of new routes or the optimization of existing routes in plants. Creative BioMart is committed to developing a variety of state-of-the-art protein engineering methods and technologies to improve crop yield and quality to alleviate global food shortages. Our researchers are very interested in discovering new and increasingly valuable solutions to various agriculture protein engineering related problems to help you be more successful. We provide the following solutions in the agriculture:

• Generating herbicide-resistant crops

Our protein engineers use directed evolution to design transferases for herbicides such as glyphosate, sulfonylureas and imidazolinones to detoxify herbicides.

• Enhancing photosynthetic efficiency

Our protein engineers enhanced the thermostability of rubisco activase (RCA) using directed evolution and introduced it into crops of interest for increased photosynthetic activity at high temperatures.

• Generating insect-resistant crops

Our protein engineers used rational and directed evolution methods to identify the sequence diversity and available 3D structures of Bt toxins, as well as receptor molecules such as cadherins, glycolipids, and aminopeptidases in the insect gut to increase Host plant resistance.

• Plant metabolic engineering

The diversity of cytochrome P450 sequences in plants and their involvement in complex metabolic pathways represent a treasure trove of genes. Our protein engineers can use protein engineering techniques to further engineer these gene pools to improve plant traits.

Our Capabilities

• Providing tailor-made solutions for the application of protein engineering in agriculture, developing stable and active enzymes, and designing gene libraries.
• Efficient, facile engineering, and robustness through protein engineering democratize agricultural applications such as improving plant productivity, disease resistance, and resilience to climate change.
• Solving problems with expertise and creativity, as well as providing complete and professional interpretation of data to understand experimental results, and better integrate external research with internal data.
• Providing unprecedented opportunity to genetically engineer new traits into next-generation crops.

Creative BioMart has created successful high-quality enzymes for the agriculture solutions for our customers. We have professionals who listen carefully to your needs and can provide you with comprehensive support before, during and after your experiment. Each project will be individually designed to meet the needs of our customers and described in a detailed project report. Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our solutions, please contact us without hesitation.

References

1. Rao A G. (2008) The outlook for protein engineering in crop improvement[J]. Plant physiology. 147(1): 6-12.
2. Engqvist M K M, Rabe K S. (2019) Applications of protein engineering and directed evolution in plant research[J]. Plant Physiology. 179(3): 907-917.

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