With the support of our advanced protein engineering platform established for many years, Creative BioMart has developed a variety of state-of-the-art techniques for evaluating protein structures modeled from amino acid sequences. Our scientists provide you with a comprehensive customized protein structure assessment service.

Protein structure prediction is achieved through X-ray crystallography or NMR spectroscopy, as well as computationally based protein modeling methods. These models are first evaluated objectively by a large number of numerical criteria. Whether the protein structure prediction is correct, or whether the spatial structure data obtained by experimental determination and structure analysis are accurate all need to be checked and judged. Computational methods for assessing proteins and their interactions have emerged in recent years and have emerged as viable alternatives to time-consuming and resource-consuming structural biology experiments in some fields. Scientists have developed a variety of assessment methods to study protein 3D structures from different perspectives, taking into account the protein's molecular environment, hydrogen bonding, secondary structure, solvent exposure, pair-residue interactions, and molecular packing.

Fig 1. Protein structure prediction pipeline. (Fischer A W, et al., 2016)

Services

Various methods (such as stereochemical examinations, energy-based molecular mechanics functions, statistical potentials, and machine learning methods) have been used to analyze the correctness of protein structures and models. Creative BioMart provides high-quality protein structure evaluation services designed to evaluate and validate protein structures generated by X-ray crystallography, NMR spectroscopy or computational modeling. The primary method of evaluation is to compare the predicted model α-carbon positions with those in the target structure. Our protein structure evaluation of the results is carried out in the following prediction categories:

• Tertiary structure prediction.
• Secondary structure prediction.
• Prediction of structural complexes.
• Residue-residue contact prediction.
• Disordered region prediction.
• Domain boundary prediction.
• functional prediction.
• Model quality assessment.
• Model refinement.
• Template-based high-accuracy prediction.

We use state-of-the-art computer software tools to perform comprehensive protein analysis by integrating sequence data and structural information. Our experienced protein engineering team can help uncover various features of target proteins, improving protein stability, affinity kinetics, solubility and activity. We provide a one-stop service for protein structure assessment:

• Protein Crystallization Service
• Protein Model Ranking and Analysis Service
• Detection of Errors in Protein Models
• Protein Conformational Isomerism Detection and Analysis Service
• Folding and Molecular Simulation Service
• Molecular Docking Service

Service Principle

Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.

We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. If you are interested in our services, please do not hesitate to contact us for more information or to discuss in detail.

Reference

1. Fischer A W, Heinze S, Putnam D K, et al.. (2016) CASP11–an evaluation of a modular BCL:: fold-based protein structure prediction pipeline[J]. PLoS One. 11(4): e0152517.

• Protein Structure Modeling Service