Creative BioMart provides Staphylococcus bacteria as a host display system service to screen out target proteins or peptide fragments from a huge random library to obtain highly active mutant enzymes or improve the immunity of antigenic determinants. With years of protein engineering research experience, we can provide you with the best quality and most professional services. If you are interested in our services, please do not hesitate to contact us for more information.

At present, there have been a large number of studies on gram-positive bacteria displaying heterologous proteins on the cell surface, and expression systems based on the fibrillar M6 protein from Streptococcus pyogenes and protein A from Staphylococcus aureus has been established. Staphylococcus is a gram-positive bacterium with a single cell membrane and an exopeptidoglycan layer that forms a rigid cell wall. Due to this strong cell wall, Staphylococcus may be suitable for industrial applications where the cell surface displays enzymes, where the bacteria will be subjected to the strong shear forces present in the bioreactor.

Creative BioMart provides Staphylococcus carnosus surface display system for affinity determination, soluble protein production and epitope positioning, etc.

Staphylococcus carnosus surface display system services

Staphylococcus carnosus has a sequence derived from Staphylococcus aureus protein A, and contains fragments required for covalent cell surface anchoring. It is widely used as a starter for sausage fermentation and contributes to food safety, flavor and controlled fermentation process. The Staphylococcus carnosus surface display system we provide can be used for the surface expression of several antigenic determinants, using live recombinant bacteria to induce local and systemic antibody responses of mixed receptors. To help you develop a live bacterial vaccine delivery system and the surface expression of single-chain antibody fragments to create a method for whole-bacterial diagnostic equipment.

The advantages of Staphylococcus surface display platforms are that can use intact bacteria for separation because the cell wall is thicker, its rigidity is stronger. And its surface receptors allow the insertion of extended sequences of foreign proteins more than different Gram-negative bacteria systems.

Fig 1. Schematic representation of the expression vector for surface display of recombinant proteins on S. carnosus. (Löfblom J, et al., 2017 )

References

1. Löfblom J. Staphylococcal surface display for protein engineering and characterization[D]. KHT, 2007.
2. Löfblom, J., Rosenstein, R., Nguyen, MT. et al.. (2017) Staphylococcus carnosus: from starter culture to protein engineering platform. Appl Microbiol Biotechnol. 101, 8293–8307.

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