With the support of our advanced protein engineering platform established for many years, Creative BioMart has developed multiple phage display platforms, including single-stranded filamentous phage (M13) display system, λ phage display system, T4 phage display system and T7 phage display system. Our scientists provide you with a comprehensive customized phage display system service for protein engineering.

Phage display technology is a screening technology widely used in protein engineering. Different foreign genes are inserted into the phage vector respectively, and as the phage is passed down, the foreign protein will be displayed on the surface of the phage to form a phage library. Subsequent high-throughput screening of the phage library with specific proteins, peptides or DNA sequences can quickly screen out the target protein or detect the interaction between the protein and other molecules. In this way, large protein libraries can be screened and amplified during the in vitro selection process.

Fig 1. The principle of phage display. (Leow C H, et al., 2017)

Advantages of Phage Display System

Phage display technology, as a new research method and tool, has been widely used in the study of protein structure. It has many significant advantages, such as:

• High-throughput screening: isolate useful genes from more than one million phage clones.
• Phage display can be used for screening of mimic epitopes, realizing the transformation between the genotype and phenotype of the substance, making it easy for people to perform a series of biochemical and genetic manipulations on it.
• Easy to purify recombinant phage：the purification steps are simple, do not require expensive reagents and equipment, and can be completed under general laboratory conditions.

Phage Display System Platform

The most commonly used phage display systems are M13, T4, T7, and λ phage. Our scientists have established a mature phage display system based on these phages for you to choose from:

• Single-Stranded Filamentous Phage (M13) Displays Service.
• λ Phage Displays Service.
• T4 Phage Displays Service.
• T7 Phage Displays Service.

Applications of Phage Display System

• Establishment of Antigen and Antibody Library.
• Drug design.
• Improving or modifing the affinity of a protein to its binding partner.
• High-throughput screening of protein interactions.
• Cell signal transduction research.
• Gene therapy.
• Vaccine research.

Service Principle

Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.

We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. Please do not hesitate to contact us for more information or to discuss in detail, if you are interested in our services.

References

1. Hoogenboom HR, de Bruïne AP, et al.. (1998) Antibody phage display technology and its applications. Immunotechnology. 4(1):1-20.
2. Leow C H, Fischer K, Leow C Y, et al. (2017) Single domain antibodies as new biomarker detectors[J]. Diagnostics. 7(4): 52.

• Cell Surface Displays Service
• Cell-Free Surface Displays Service