With the support of our advanced protein engineering platform established for many years, Creative BioMart has developed multiple cell surface display platforms, including bacteria surface display system, yeast surface display system, baculovirus-insect cell display system and mammalian cell display system. Our scientists provide you with a comprehensive customized cell surface display system service for protein engineering.

The cell surface display technology is to introduce the target protein (polypeptide or protein) gene sequence into the cell after fusion with the specific carrier protein gene sequence, so that the target protein is displayed on the surface of the microbial cell. Common carrier proteins include bacterial outer membrane proteins, extracellular accessory structures (such as flagella) proteins, etc. Commonly used host cells include bacteria and yeast cells. Cell surface library sizes of up to 1011 transformants have been reported with each cell displaying thousands of identical copies of a particular mutant protein. High-throughput screening of the library was carried out through a variety of methods, and cells showing proteins with the expected phenotype were Separate for cultivation and reproduction. The characteristics of the carrier protein, target protein and host cell, as well as the fusion method, will affect the efficiency of protein surface display. In this way, it is often used to screen the interaction between protein and protein, especially the interaction between antigen and antibody.

Fig 1. Cell surface protein engineering for high-performance whole-cell catalysts. (Nakatani, et al., 2017 )

Advantages of Cell Surface Display System

Although due to the fragility of the cell membrane and other issues, cell display is not as widely used as phage display. But it has the following advantages:

• The large size of bacteria and yeast cells allows them to be screened using fluorescence microscopy or flow cytometry.
• The size of the foreign protein to be displayed is quite limited on the surface of the phage. The cell surface display system was developed to solve this problem.
• Cell surface display allows easy adjustment of library screening conditions, such as buffer composition, pH value, and ionic strength. It usually requires multiple rounds of screening to obtain a rich library of mutants with the desired phenotype.
• Cell surface display can quickly characterize individual mutants without the need for soluble protein expression and purification.

Cell Surface Display System

The cell surface display system is used to express proteins or peptides on the cell surface of prokaryotes and eukaryotes (such as bacteria, yeast, insect and mammalian cells). Our scientists have established a mature cell surface display system based on these cells for you to choose from:

• Bacteria Surface Displays Service.
• Yeast Surface Displays Service.
• Baculovirus-Insect Cell Displays Service.
• Mammalian Cell Displays Service.

Applications of Cell Surface Display System

• Live vaccine development.
• Peptide library screening.
• Biotransformation and biosorption using whole-cell biocatalyst.
• Protein engineering.

Service Principle

Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.

We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. Please do not hesitate to contact us for more information or to discuss in detail, if you are interested in our services.

References

1. Lee SY, Choi JH, Xu Z. (2003) Microbial cell-surface display. Trends Biotechnol. 21(1): 45-52.
2. Nakatani, H., Hori, K. (2017) Cell surface protein engineering for high-performance whole-cell catalysts. Front. Chem. Sci. Eng. 11, 46–57.

• Cell-Free Surface Displays Service
• Phage Displays Service