With the support of our advanced protein engineering platform established for many years, Creative BioMart has adopted a bottom-up rational design strategy to achieve complete redesign of multiple proteins by using a knowledge-based approach to tune the parameters of the energy function. Our scientists provide you with a comprehensive customized self-assembly of segments of secondary structure service.

Secondary structure formation as a feature of polypeptides is fundamental to the higher ordered structural diversity and specific functions of proteins and other biological materials. The structural features of peptides are mainly amide bonds, allowing them to spontaneously self-assemble, which is mainly driven by non-covalent interactions such as hydrogen bonding, electrostatic attraction, and van der Waals forces. Two types of secondary structure elements are used in peptide self-assembly, α-helix and β-sheet, but random coil is also sometimes used. The most common secondary structure found in globular proteins is the alpha-helix, and self-assembling peptides are usually designed from the native sequence of the helical protein or by incorporating non-coding amino acids with higher helical propensity into the sequence. It is much easier to modify and control the structure of peptide-based assemblies than protein-based assemblies. Self-assembled peptides have been used for a wide range of applications, including new biomaterials, surface coating materials and semiconductor devices, as well as novel antibiotics for combating drug resistance problems.

Fig 1. The pathway in biological systems for synthesis of sophisticated proteins and the strategy of peptide tectonics to create comparable bioinspired or biomimetic systems via rational control over the interfacial association between structural complementarity at predictable interfaces. (Lou S, et al., 2019)

Services

Synthetic peptides derived from native protein subunits or created by de novo design have been used as building blocks for self-assembly to construct various complex structures and develop functional materials from the bottom up. Creative BioMart has successfully created an extensive library of peptides with desirable biochemical (reactivity, chirality and toxicity) and physical (size, shape and conformation) properties. Based on a modular approach, we sought to engineer individual peptide segments of α-helices or β-sheets to self-assemble with several identical copies to generate novel supramolecular structures. In addition, peptides are attractive components due to their facile synthesis, rich chemical diversity, and inherent biocompatibility, and we work on the self-assembly of peptides and their various analogs for the preparation of various interesting of nanostructures, including fibers, tubes, ribbons, vesicles, films, and three-dimensional (3D) networks.

Peptides are attractive components due to their facile synthesis, rich chemical diversity, and inherent biocompatibility. We work on the self-assembly of peptides and their various analogs for the preparation of a variety of interesting materials. Successfully designed general-purpose peptide materials include:

(1) Lego-like peptides: they self-assemble to form nanofibers.

(2) Lipid-like or surfactant-like peptides: they self-assemble into nanotubes and nanovesicles.

(3) Biopaint-like peptides: they self-assemble on surfaces to modify surfaces at the nanoscale.

Customized Service

Additionally, based on your needs, we tailor peptide materials to meet specific research or application needs by attaching specific active motifs.

• For tissue engineering, wound healing, addressing chronic wounds and regenerative medicine.
• Accelerates wound healing in human clinical and surgical applications.
• Sustained molecular release (small molecules, protein growth factors and monoclonal antibodies).
• Stabilization of various membrane proteins for nanobiotechnology device fabrication in solution and dry surfaces, and production of G protein-coupled receptors.

Service Principle

Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.

We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. If you are interested in our services, please do not hesitate to contact us for more information or to discuss in detail.

References

1. Zhang S. (2017) Discovery and design of self-assembling peptides[J]. Interface focus.7(6): 20170028.
2. Lou S, Wang X, Yu Z, et al.. (2019) Peptide Tectonics: Encoded Structural Complementarity Dictates Programmable Self‐Assembly[J]. Advanced Science. 6(13): 1802043.

• Knowledge-Based Membrane Protein Design
• Knowledge-Based Novel Protein Folds Design
• Knowledge-Based Preorganized Structure Design
• Knowledge-Based Single-Chain Protein Design