With our extensive experience, we are able to provide custom gene amplification services using advance techniques. We offer a wide range of services which are highly targeted and focused, including polymerase chain reaction gene amplification, ligase chain reaction gene amplification, transcription-mediated gene amplification and natural gene amplification. By using these methods, our experts can accurately verify, identify and amplify genes in protein engineering. Our services are cost-effective and punctual.

In directed evolution strategies, the last step is to select the best mutation sequence or sequence pool for gene amplification. Using the best sequence, the entire cycle of mutagenesis, screening, selection, and gene amplification is repeated until a mutant with the characteristics required by the protein engineering target is identified. Gene amplification is a process in which the copy number of a gene encoding a specific protein is selectively increased while other genes are not increased proportionally. There are two main ways of gene amplification: artificial gene amplification and natural gene amplification. This technology can specifically amplify a very small amount of target DNA millions of times, thereby greatly improving the analysis and detection capabilities of DNA molecules. Therefore, this method is widely used and rapidly developed in many fields such as molecular biology, microbiology, medicine and genetics.

Fig 1. Selective gene amplification scheme. (Kelly BT, et al., 2007)

Creative BioMart can provide the most affordable gene amplification service with the fastest turnaround time to meet your downstream needs. Creative BioMart provides protein engineering gene amplification services for your scientific research based on the following gene amplification technologies:

• Polymerase Chain Reaction (PCR) Technology

We provide a simple, cheap, and reliable polymerase chain reaction gene amplification service that replicates target DNA fragments by polymerizing nucleotides repeatedly.

• Ligase Chain Reaction (LCR) Technology

We provide a ligase chain reaction gene amplification service that obtains probes by amplifying nucleic acids, including DNA polymerase used by LCR (for initial template amplification) and thermostable DNA ligase.

• Transcription-mediated Amplification Technology

We provide transcription-mediated gene amplification services through isothermal gene amplification methods, using RNA polymerase and reverse transcriptase to rapidly amplify target RNA/DNA.

• Natural Gene Amplification Technology

We provide natural gene amplification services that can replicate any DNA fragment containing genes. Common sources of gene duplication include ectopic recombination, retrotransposition events, aneuploidy, polyploidy, and replication slippage.

If you have any special requirements about our gene amplification services for protein engineering, please feel free to contact us. We are looking forward to working together with your attractive projects.

Reference

1. Kelly BT, Griffiths AD. (2007) Selective gene amplification. Protein Eng Des Sel. 20(12): 577-581.

• Protein High-Throughput Screening Services
• Protein Mutation Library Construction Services
• Successful Protein Engineering from Utilizing Directed Evolution