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With the support of our advanced protein engineering platform that we have established for many years, Creative BioMart has successfully designed a variety of platforms for different applications to produce special proteins based on the directed evolution strategy. Creative BioMart offers customers design and engineering services for synthetic binding proteins using nonantibody scaffolds, which can accommodate a large number of sequence changes (substitutions, insertions and deletions) to generate the required functions.
In recent years, people have paid extensive attention to the production of new classes of ligand-specific binding proteins to supplement monoclonal antibodies. The combination of protein engineering and display technology has been used to manipulate non-human antibodies for humanization and stabilization purposes, and even to produce new binding proteins. The synthetic binding protein is constructed using a non-antibody molecular scaffold. The advantageous properties of synthetic binding proteins, such as small size, no disulfide bond formation, and easy preparation of fusion proteins, make it unique in the fields of protein science and cell biology.
Fig 1. Recent Advances in the Scaffold Engineering of Protein Binders. (Ahmadi MKB, et al., 2021 )
Engineering binding proteins derived from non-antibody scaffolds are becoming more prominent in research and therapeutic applications. Creative BioMart uses various protein domains as non-antibody scaffolds to produce synthetic binding proteins that have the ability to bind to molecules. We mainly provide two types of non-antibody scaffolds for you to choose from: domain-sized compounds (Affibodies, Affilins, Anticalins, Atrimers, DARPins, FN3 scaffolds, Fynomers, Kunitz domains and OBodies) and constrained peptides (Avimers, Bicyclic peptides and Cys-knots). After verification, it was found that these protein scaffolds have shown great potential in terms of affinity, target neutralization and stability, allowing peptides with affinity for a specific target to be fused or inserted with the carrier protein, so that their binding properties are combined with the desired properties of the scaffold.
Creative BioMart can help you design various binding proteins to achieve proper folding and stability. In addition, we can also provide engineering protein synthesis and structure determination, and evaluate its properties through relevant biochemical and/or biophysical techniques. We provide different customized non-antibody scaffold synthetic binding protein design services, including but not limited to:
Our non-antibody scaffold synthetic binding protein has a wide range of potential applications, including research tools, dissociation omics, diagnosis and therapy. For example, effective tissue penetration for the treatment of solid tumors, intracellular activity of targeted cell signaling pathways, modifications that are easy to fuse with effector molecules, improved physical and chemical stability, and cost-effective manufacturing processes.
Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.
We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. Please do not hesitate to contact us for more information or to discuss in detail, if you are interested in our services.
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