With our extensive experience, we are able to provide customized successful protein engineering paltform to engineer your protein using directed evolution methods. We offer a wide range of services which are highly targeted and focused, including using non-antibody scaffolds to design and modify synthetic binding proteins, enzyme engineering, therapeutic protein engineering, engineered biomaterials and protein engineering using noncanonical amino acids. By using these methods, our experts can design proteins with more complex functions for new applications.

Natural evolution has produced a wide variety of proteins that can be used in numerous applications in biotechnology and pharmaceutical sciences. Usually, specific applications require protein engineering to customize proteins. Directed evolution is a type of protein engineering that can produce proteins with desired characteristics under clearly defined conditions and actual time ranges. We have provided protein mutation library construction services, protein high-throughput screening services, and gene amplification services to produce proteins that meet your needs based on directed evolution strategies. However, designing protein applications usually requires complex and subtle functions.

With the continuous development of the field of life sciences, higher requirements are put forward for protein engineering. With the support of the most comprehensive and professional protein engineering platform and years of accumulated experience, Creative BioMart has successfully carried out protein engineering using directed evolution strategies to provide proteins with more complex structures and finer functions for your applications.

Fig 1. Schematic view of the various steps of a directed evolution campaign. (Gargiulo S, et al., 2021)

Creative BioMart guarantees the fastest turnaround time to provide the most affordable and successful protein engineering using directed evolution meet your downstream needs. Creative BioMart's successful protein design utilizing evolution platform includes but is not limited to:

• Design and Engineering of Synthetic Binding Proteins Using Nonantibody Scaffolds

Highly specific molecular recognition is one of the basic functions of proteins. Our service is possible by using non-antibody scaffolds to create new molecular recognition sites on other proteins, providing customers with design and engineering services for synthetic binding proteins. We can help you design proteins with various existing scaffolds, taking into account factors such as disulfide bond formation and H bonding to achieve proper folding and stability.

• Enzyme Engineering

Enzyme activity (catalytic conversion of the substrate) needs to be maintained or possibly increased by mutagenesis, which poses an important challenge to the screening and selection system that distinguishes enzyme engineering and protein binding engineering. Therefore, our combined method applied to enzyme engineering modifies these technologies applied to the engineering properties of non-catalytic proteins, allowing you to choose the threshold level of enzyme activity required to monitor catalysis or cell survival.

• Engineering of Therapeutic Proteins

There are two main types of drugs on the market: small molecule drugs and protein therapies. We develop proteins with the clinical potential to enhance efficacy and safety, reduce immunogenicity, and improve delivery.

• Protein Engineered Biomaterials

We use recombinant protein technology to design and synthesize a variety of protein-engineered biomaterials that achieve precise control over macromolecular composition and function, with tunable mechanical, biochemical, and biodegradable properties.

• Protein Engineering Using Noncanonical Amino Acids

Methods of engineering proteins containing non-canonical amino acids are developing rapidly. We introduce new amino acids into recombinant proteins in a residue-specific or site-specific manner to engineer the overall physical and chemical behavior of proteins and protein-like macromolecules.

If you have any special requirements about our successful protein engineering platform using directed evolution methods, please feel free to contact us. We are looking forward to working together with your attractive projects.

Reference

1. Gargiulo S, Soumillion P. (2021) Directed evolution for enzyme development in biocatalysis. Curr Opin Chem Biol. 61:107-113.

• Gene Amplification Services
• Protein High-Throughput Screening Services
• Protein Mutation Library Construction Services