Backed by our years-established advanced protein engineering platform, Creative BioMart can provide you with a comprehensive, customizable protein expression service in insect cells with strong expertise in protein expression.

The insect cell expression system belongs to the eukaryotic cell expression system and is one of the commonly used expression systems. A variety of exogenous genes can be expressed in insect cells. The principle is to first place the expression components in the transfer vector on the baculovirus shuttle vector of Escherichia coli by transposition, and then screen by resistance and blue-white classes into the recombinant shuttle plasmid. Then the plasmid is extracted and transfected into insect cells to obtain progeny virus. Finally, the virus supernatant was used to infect insect cells to obtain the final recombinant protein. Compared with other expression systems, insect expression systems have many advantages and can perform translation processing modifications such as glycosylation, acetylation, and phosphorylation of proteins. In insect cell expression systems, exogenous genes are expressed at high levels, especially intracellular proteins. The system itself is easy to operate, and insect cells grow in suspension, which is conducive to the large-scale expression of recombinant proteins. Insect baculovirus itself is a kind of double-stranded DNA virus that uses insect cells as its natural host. It has a high degree of species specificity, does not infect vertebrates, and has high biological safety.

Fig 1. The flowchart of recombinant protein expression in insect cells. (Chang, J. C, et al., 2018)

Service Content of Protein Expression in Insect Cells

• Gene Synthesis and Codon Optimization. The customer needs to provide the sequence information or plasmid of the target protein, and we design the relevant primer sequences according to the customer's experimental needs, complete the codon optimization and gene synthesis, and finally introduce the target gene into the appropriate vector.
• Construction of the Vector. We select an appropriate vector and clone the target gene into an expression vector to construct a recombinant vector. And also provide plasmid preparation, plasmid sequencing, and other services.
• Virus Packaging and Amplification. The extracted recombinant plasmids were transfected into insect cells, and the P1 and P2 generation viruses were collected and amplified.
• Expression and Purification of Target Proteins. The target protein is expressed in small amounts by infecting insect cells such as Sf9 with a transfection reagent, and then the target protein is purified by nickel column, GST column, and other methods. All purified proteins are subject to rigorous QC (SDS-PAGE, UV, etc.) analysis.
• Protein Expression Optimization. We will optimize the conditions of protein expression time, the multiplicity of infection (MOI), virus titer, etc., to provide you with the best service for protein expression in insect cells.
• Large-Scale Protein Expression and Purification. We can provide you with large-scale protein expression and purification services of 100 mg or more protein according to your needs for purified protein.

Deliverables

• Sequencing report
• Purified protein
• Quality inspection report
• Experimental report

Our Advantages

• Provide targeted test programs to meet all customer needs.
• We can provide protein expression and purification services on different scales.
• With powerful experimental facilities and rich experience in protein expression, we ensure that expression service is completed quickly and efficiently.

Creative BioMart offers a professional and cost-effective service for expressing proteins in insect cells, and we are waiting for you 24 hours a day. If you have any interest in this service with us, please feel free to contact us for more information or to discuss it in detail.

Reference

1. Chang, J. C., et al. (2018). Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay. Journal of visualized experiments: JoVE, (132), 56693.

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