Creative BioMart is a well-known expert specializing in the application of advanced cell-free display technology to accelerate the release of protein engineering. With years of experience, we are able to provide mRNA display system service based on cell-free technology to precisely meet customer requirements.

The mRNA display technology is an emerging powerful tool for screening peptides and proteins in vitro, which can discover and directed evolution of new molecules from combinatorial libraries. In mRNA display, the mRNA molecule covalently links the displayed peptide or protein (phenotype) with the encoded genetic information (genotype) through the biochemical activity of a small molecule of puromycin. These mRNA-protein fusions can select >10¹³ different sequences in vitro. mRNA display has been used to discover novel peptides and protein ligands for RNA, small molecules and proteins, as well as to define cellular interaction partners of proteins and drugs. In addition, mRNA display can also achieve a variety of unique applications, including self-assembled protein chips and library construction using unnatural amino acids and chemically modified peptides.

Services

Creative BioMart provides the covalent linkage of phenotype (protein) and genotype (RNA) in the mRNA display technology  effectively allows the protein to be directly amplified, which can be screened in a large-capacity peptide library (10¹³～10¹⁵) peptides and proteins with specific biological functions. The size of this library is much larger than most in vivo and in vitro selection methods, and several orders of magnitude larger than those used in traditional screening techniques. As an in vitro display technology, our mRNA display system is popular because of its large peptide library capacity, high screening efficiency, and easy operation.

The mRNA display technology we provide is mainly used to discover new protein ligands such as RNA, small molecules, and proteins, and to clarify the interaction mechanism between proteins and drugs in cells. In addition, it can also provide comprehensive solutions for self-assembled protein chips, libraries of unnatural amino acids, and chemical modification of peptides.

Fig 1. Directing evolution of novel ligands by mRNA display. (Kamalinia G, et al., 2021)

Features of mRNA Display System

• The phenotype-genotype linkage in mRNA display is covalent and highly stable, allowing multiple types of selection under very strict conditions.
• mRNA display uses a cell-free translation system to generate peptide sequences, and expression and selection are performed in vitro. A peptide or protein library containing up to 10¹²-10¹⁴ can be easily generated and selected for unique sequences.
• mRNA display allows the use of any candidate library with independent random regions or in the context of the desired fusion protein.
• We use mRNA-displayed proteomic libraries, whose peptides are in the range of 50-300 residues in length, and are more likely to adopt natural conformations or structures than short synthetic peptides.
• mRNA display uses PCR to amplify cDNA in each round of selection. The resulting PCR product is directly used for in vitro transcription and translation, making it highly compatible with PCR-based mutagenesis and recombination technology.

Creative BioMart has been continuously developing cutting-edge cell-free display technology, and is committed to providing a flexible and efficient customized mRNA display system service. Based on the protein engineering platform, we have successfully constructed an impeccable cell-free surface display library of various types. We are proud to be able to screen and select proteins with the required characteristics from a large number of variants to best accelerate the research of life sciences. If you are interested in our services, please do not hesitate to contact us for more information.

References

1. Wang H, Liu R. (2011) Advantages of mRNA display selections over other selection techniques for investigation of protein-protein interactions. Expert Rev Proteomics. 8(3): 335-346.
2. Kamalinia G, Grindel BJ, et al.. (2021) Directing evolution of novel ligands by mRNA display. Chem Soc Rev. 50(16): 9055-9103.

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