Nanoparticles find wide-ranging applications in biotechnology, materials, nanoelectronics, cancer therapy and imaging, environmental and biofuel industries. With its advanced protein engineering platform established over the years, Creative BioMart uses a variety of proteins to design protein nanoparticles for drug delivery, nanomaterial synthesis, vaccines and biocatalysis. Our scientists are committed to providing fully customized protein-based nanoparticles solutions to customers around the world.

Introduction of Protein-Based Nanoparticles

Current nanocarriers include inorganic nanoparticles (such as iron oxide, quantum dots, gold nanoparticles) and organic nanoparticles (such as liposomes and dendrimers). However, they are limited by their structural instability and potential toxicity. Therefore, in order to enhance their therapeutic effect, it is necessary to design nanoparticles that can release drugs in response to stimuli. Protein nanoparticles stand out among nanocarriers due to their biocompatibility, biodegradability, and low immunogenicity. In addition, their preparation conditions are mild and do not require toxic solvents. These properties provide a basis for the design and construction of novel protein nanoparticles using native oligomeric proteins and/or computer-designed protein sequences. Studies have found that protein nanoparticles as drug carriers can increase the therapeutic effect by increasing pharmacokinetics, prolonged controlled release, local delivery, and drug release. Furthermore, protein nanoparticles have been developed as ideal carriers for cancer therapy to reduce drug toxicity and improve therapeutic efficacy.

Fig 1. The schematic illustration of protein nanoparticles directed cancer imaging and therapy. (Miao Y, et al., 2022)

Solutions

Protein-based nanoparticles exhibit complex but genetically modifiable protein structures. Combining this information obtained with new computational tools for molecular design and engineering makes PNPs ideal candidates for rational redesign. Creative BioMart has successfully constructed nanoparticle platforms, including fibroin, albumin, gelatin, gliadin, soy protein, 30Kc19, lipoprotein and ferritin, etc. In addition, our protein engineers utilize rationally designed protein engineering strategies to enhance or alter the natural properties of nanoparticles to meet the requirements of specific applications, such as stability, structural asymmetry, biocompatibility, etc. In order to provide customized high-quality protein nanoparticles to global customers, we choose the best materials or processes to provide solutions according to specific situations, including but not limited to:

• Identification of protein nanoparticles.
• Production of protein nanoparticles.
• Purification of protein nanoparticles.
• Rational design of protein-based nanoparticles.
• Predicting the effect of protein modification on the structural stability of nanoparticles and their self-assembly ability.
• Drug loading of protein-based nanoparticles.
• Drug release from protein-based nanoparticles.

Advantages of Protein-Based Nanoparticles

• Protein nanoparticles have three different interfaces that can be designed to acquire new functions (internal, external, and inter-subunit.
• Crystal structure, genetic and molecular information is available for many protein nanoparticles, allowing rational chemical and/or genetic modification.
• Compared with other colloidal carriers, protein nanoparticles have the advantages of being more stable and easier to manufacture.
• Proteins from various sources can be made into nanoparticles through a simple, economical and environmentally friendly synthesis process while using fewer chemicals.
• Protein nanoparticles can improve protein transfer efficiency by controlling properties such as size, shape, and surface charge.
• Adjust protein drug loading and release efficacy according to the properties of nanoparticles or the concentration and type of drugs.

Creative BioMart has in-depth knowledge and experience of the tools and processes involved in nanoparticles development, and provides professional protein-based nanoparticles solutions for the customers. Whether your protein-based nanoparticles are in the discovery stages, or are planning engineered protein-based nanoparticles production, please contact us to discuss further details to ensure your next success.

References

1. Diaz D, Care A, Sunna A. (2018) Bioengineering strategies for protein-based nanoparticles[J]. Genes. 9(7): 370.
2. Miao Y, Yang T, Yang S, et al.. (2022) Protein nanoparticles directed cancer imaging and therapy[J]. Nano Convergence. 9(1): 1-21.

• Protein-Based Nanofibers
• Protein-Based Nanotubes