Creative BioMart's strong expertise in high-throughput screening of directed evolution of proteins enables us to accelerate your project by providing protein for expression of different mutant genes screening services , including phage display system, cell surface display system, cell-free display system, flow cytometry sorting technology. This service can perform high-throughput screening of a huge mutant library, so you can select the mutant strain that meets your needs.

In directed evolution strategies, the keys to success are the diversity of mutant libraries and the establishment of high-throughput screening methods. Our mutation library construction services are quite mature, with up to 1011 mutants. The larger the mutant library and the greater the number of mutants, the higher the chance of screening the desired mutants. For these huge mutant libraries, high-throughput screening is necessary. Choosing a suitable screening system can quickly screen out the target protein from the mutant library.

Fig 1. Using library-based display technologies to evolve protein stability. (Ren, C., et al., 2019)

Creative BioMart can provide the most affordable protein high-throughput screening service with the fastest turnaround time to meet your downstream needs. Creative BioMart provides the following protein for expression of different mutant genes screening services for your scientific research:

• Phage Display Technology

The phage display technology is to insert the polypeptide or protein coding gene into the appropriate position of the phage coat protein structural gene, so that the foreign gene is expressed along with the expression of the coat protein. At the same time, the foreign protein is displayed on the surface of the phage as the phage is reassembled.

• Cell Surface Display Technology

The cell surface display technology is to fuse a foreign protein gene sequence with a specific carrier protein gene sequence and then introduce it into the cell, so that the foreign protein is expressed and positioned on the cell surface.

• Cell-Free Display Technology

Phage display systems and cell surface display systems require the participation of living biological cells. Due to the limitation of transformation efficiency, each transformation will cause the loss of mutation library diversity. Cell-free display technology provides an alternative to host-dependent methods for directed evolution of proteins.

• Flow Cytometry Sorting Technology

Fluorescence activated cell sorting technology is a technology for rapid quantitative analysis of cells or other tiny biological particles. The proteins displayed on the surface of microbial cells are screened by flow cytometry by adding fluorescent ligands or substrates.

If you have any special requirements about our construction services of protein mutation library, please feel free to contact us. We are looking forward to working together with your attractive projects.

References

1. Ren, C., Wen, X., Mencius, J. et al.. (2019) Selection and screening strategies in directed evolution to improve protein stability. Bioresour. Bioprocess. 6, 53.
2. Wójcik M, Telzerow A, Quax WJ, et al.. (2015) High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives. Int J Mol Sci. 16(10): 24918-45.

• Gene Amplification Services
• Protein Mutation Library Construction Services
• Successful Protein Engineering from Utilizing Directed Evolution