Peroxidases have broad application prospects as green catalysts in bioremediation and have considerable potential in energy conversion. With its advanced protein engineering platform established over the years, Creative BioMart has successfully developed engineered peroxidases have the ability to maintain activity under extreme pH and temperature conditions, high catalytic performance and stability. Our scientists are committed to providing fully customized engineered peroxidases production solutions for environment to customers around the world.

Introduction of Peroxidases Production

The fast-growing consumer demand has promoted the development of industry, but it has also caused environmental pollution. Peroxidases are a class of oxidoreductases capable of carrying out a wide variety of oxidative reactions. Peroxidase is currently an interesting alternative in cleaning the environment, and a variety of plant peroxidases are used in bioremediation, such as the elimination of synthetic dyes in the textile industry and the polymerization of phenolic compounds. Furthermore, the sensitivity and selectivity of peroxidase-based biosensors were exploited for component quantification and development of diagnostic kits. Despite the effectiveness of peroxidases in the degradation of harmful compounds, broad application in free-form environmental remediation cannot be envisaged due to their lack of handling and storage stability. To overcome these drawbacks, protein engineering is used for the modulation and immobilization of peroxidase structures. For example, researchers have successfully developed a simplified directed evolution workflow to accelerate the protein engineering of dye-decolorizing peroxidase, showing great promise in industrial biocatalysis and lignocellulose degradation.

Fig 1. A rational engineering approach to design multifunctional plant-fungal hybrid peroxidases that can function in a wider temperature and pH range. (Kohler A C, et al., 2018)

Solutions 

As biocatalysts, peroxidases perform bioremediation of various potentially hazardous compounds and play an important role in improving environmental quality. Creative BioMart has successfully utilized a variety of protein engineering techniques to produce engineered peroxidases that are stable under extreme pH and temperature conditions. The peroxidases we designed are fully in line with the ambition of economic transformation from the classical model to a system based on a circular economy. In order to provide customized high-quality peroxidases to global customers, we take the following solutions:

• Controling peroxidase substrate specificity.
• Identification of residues in the aromatic substrate binding site of peroxidase.
• Improving the stability of this peroxidase at extreme pH and temperature.
• Protecting oxidation-sensitive residues in peroxidases, or design more efficient intramolecular free radical partition pathways.
• Looking for hydrogen peroxide substituents, especially in hydrophobic organic media.

Applications of Protein Engineering in Peroxidases Production

Protein engineering has stabilized, or even improved, the catalytic properties of peroxidases, resulting in significantly lower costs for the desired applications. Engineered peroxidases have emerged as powerful tools for going green, eliminating environmentally persistent aromatic hydrocarbons in different types of media without generating additional waste. We employed rational design combined with directed evolution to alter the properties of peroxidases.

• Structure-Based Molecular Modeling Approaches
  Our protein engineers improve peroxidase stability by engineering the peroxidase active site to replace amino acids susceptible to oxidative damage.
• Random mutagenesis
  After successive rounds of random mutagenesis and screening of peroxidases, our protein engineers obtained mutants with increased peroxidase activity, modifying their catalytic properties.
• Site-directed mutagenesis
  Our protein engineers use site-directed mutagenesis to target amino acid residues susceptible to hydrogen peroxide oxidation, and identified and combined three amino acids to create a variant that improved the protein's stability to hydrogen peroxide.

Creative BioMart has in-depth knowledge and experience of the tools and processes involved in engineered laccases development, and provides professional laccases production solutions for the environment. Whether your laccases are in the discovery and screening stages, or are planning engineered laccases production, please contact us to discuss further details to ensure your next success.

References

1. Sellami K, Couvert A, Nasrallah N, et al.. (2022) Peroxidase enzymes as green catalysts for bioremediation and biotechnological applications: A review[J]. Science of the Total Environment. 806: 150500.
2. Kohler A C, Simmons B A, Sale K L. (2018) Structure-based engineering of a plant-fungal hybrid peroxidase for enhanced temperature and pH tolerance[J]. Cell chemical biology. 25(8): 974-983. e3.

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