Haloalkane dehalogenases are potential catalysts for biodegradation of some environmentally toxic substances discharged as industrial by-products. With its advanced protein engineering platform established over the years, Creative BioMart has successfully improved the properties of haloalkane dehalogenases, including activity, thermostability, substrate specificity and enantioselectivity. Our scientists are committed to providing fully customized engineered haloalkane dehalogenases production solutions for environment to customers around the world.

Introduction of Haloalkane Dehalogenases Production

Haloalkanes are serious environmental pollutants. The elimination of toxic haloalkanes is very important, and the use of haloalkane dehalogenases is a promising and environmentally friendly way to achieve this. Haloalkane dehalogenase is an ɑ/β hydrolase that converts halogenated compounds to the corresponding alcohols, halides and protons. Due to its unique catalytic mechanism and broad substrate specificity, haloalkane dehalogenases have been used in a variety of applications in the environment, including bioremediation of environmental pollutants, recovery of by-products of chemical processes, decontamination of chemical warfare agents, etc. Currently, the application of haloalkane dehalogenases is limited due to low expression of haloalkane dehalogenases, product inhibition, insufficient enzyme selectivity, low affinity for selected substrates and low catalytic efficiency, and instability in the presence of organic co-solvents. The advent of the era of protein engineering has improved the thermal stability of haloalkane dehalogenases, and attempts to improve their activity by semi-rational methods have been successful. Three typical haloalkane dehalogenases, namely DhlA, DhaA and LinB, have been intensively studied through biochemical, structural and computational analyses.

Fig 1. Substrate specificity and enantioselectivity of haloalkane dehalogenases. (Koudelakova T, et al., 2013)

Solutions 

Haloalkane dehalogenases are considered as key enzymes in the degradation pathway of halogenated compounds synthesized by pollutants, and play an important role in improving environmental quality. Creative BioMart has successfully utilized a variety of protein engineering techniques to produce engineered haloalkane dehalogenases with activity, thermostability, substrate specificity and enantioselectivity. In order to provide customized high-quality haloalkane dehalogenases to global customers, we present possible solutions from strategies based on protein engineering and the isolation of novel haloalkane dehalogenases from extremophiles.

• Enhancing enantioselectivity of haloalkane dehalogenases.
• Enhancing resistance of haloalkane dehalogenases to organic solvents for purification purposes.
• Increasing the activity of haloalkane dehalogenases or modify the specificity of substrates for bioremediation.
• Expandeding pH range for haloalkane dehalogenase biosensing.
• Increasing thermostability and long-term stability of haloalkane dehalogenases in various applications.

Applications of Protein Engineering in Haloalkane Dehalogenases Production

Early protein engineering research focused on the rational selection of potential catalytic residues in haloalkane dehalogenases for site-directed mutagenesis based on X-ray structures or homology models. Current protein engineering studies examine the possibility of improving the catalytic efficiency or stability of haloalkane dehalogenases. Our solutions will provide biocatalysts that meet practical needs.

• Computational design-based approaches
  Our protein engineers used computational design-based methods to modify the entry channel to create haloalkane dehalogenase mutants with altered activity and specificity.
• Directed evolution approaches
  Our protein engineers screen single-site mutants of haloalkane dehalogenases using high-throughput activity assays, resulting in haloalkane dehalogenase mutants with improved stability and enantioselectivity.

Creative BioMart has in-depth knowledge and experience of the tools and processes involved in engineered haloalkane dehalogenases development, and provides professional haloalkane dehalogenases production solutions for the environment. Whether your haloalkane dehalogenases are in the discovery and screening stages, or are planning engineered haloalkane dehalogenases production, please contact us to discuss further details to ensure your next success.

References

1. Koudelakova T, Bidmanova S, Dvorak P, et al.. (2013) Haloalkane dehalogenases: biotechnological applications[J]. Biotechnology journal. 8(1): 32-45.
2. Nagata Y, Ohtsubo Y, Tsuda M. (2015) Properties and biotechnological applications of natural and engineered haloalkane dehalogenases[J]. Applied microbiology and biotechnology. 99(23): 9865-9881.

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