The key properties of enzymes for industrial applications are activity, thermostability, solvent stability, and substrate specificity. Protein engineering is an important tool for meeting the needs of enzymes adapted to different industrial processes. Based on our extensive experience in protein engineering, researchers can range from experimental protein construction and theoretical protein design to industry applications of engineered proteins. Creative BioMart is committed to developing a variety of industrial enzymes with improved activity, specificity and stability for bioremediation, biofuels and detergents, etc. We have the ability to provide global customers with the best solutions for protein engineering applications in industry.

Background

In industrial applications, enzymes are more prominent than traditional chemistry because of their higher reaction rate, specificity, product yield, product quality, and operational safety in catalytic reactions. In addition, enzymes can reduce energy and chemical consumption. These properties can be used to synthesize high value-added products. Therefore, enzymes play an important role in promoting the development of green industry. Studies have found that enzymes can be used in biofuel production because they can degrade a range of substrates to produce ethanol and biodiesel. Bioremediation with enzymes has several advantages, including easier control and safer, easier handling. In addition, the use of enzymes in detergents can replace harmful compounds as well as reduce energy consumption and the release of environmental pollutants. However, the natural strength of many enzymes is insufficient for industrial use. The development of protein engineering has subsequently enabled enzymes to meet the different needs of different industrial sectors, promoting industrial enzymology and its applications. For example, scientists have successfully applied site-directed mutagenesis to add two disulfide bonds to recombinant xylanase, thereby significantly increasing its temperature adaptation.

Fig 1. Challenges in choosing the best approach for enzyme engineering to obtain improved enzymatic properties. (Victorino da Silva Amatto I, et al., 2021)

Custom Solutions for the Industry

In order to overcome the limitations of wild-type enzymes and make them suitable for industrial use, it is necessary to adopt protein engineering techniques to change their amino acid sequences to improve their specific properties. Creative BioMart is committed to developing a variety of state-of-the-art protein engineering methods and technologies to improve key properties of industrial enzymes including activity, thermostability, solvent stability and substrate specificity, thereby promoting green industry development. Our researchers are very interested in discovering new and increasingly valuable solutions to various industry protein engineering related problems to help you be more successful. We provide the following solutions in industry:

• Nitrilases Production Solutions

The application of nitrilases as important biocatalysts in nitrile degradation has attracted considerable attention. Our protein engineers use a directed evolution approach to improve the catalytic properties of nitrilase enzymes, such as specific activity, substrate tolerance, and thermostability.

• Aldolases Production Solutions

Aldolase is an important enzyme for carbon-carbon bond formation in stereoselective synthetic reactions in synthetic organic chemistry. Our protein engineers use protein engineering or screening methods to improve aldolase synthesis reactions.

• Xylanases Production Solutions

Xylanases are widely used in the pulp and paper industry. Our protein engineers use rational and random mutagenesis strategies to enhance the thermostability of this industry-relevant enzyme. Furthermore, mutant xylose-tolerant xylanases were designed using error-prone PCR directed evolution.

Our Capabilities

• Providing tailor-made solutions for the application of protein engineering in industry, developing industrial enzymes with the following properties: long half-life, use of non-natural substrates, retention of activity under extreme conditions such as pH and temperature, weatherability to organic solvents, enantioselectivity, and optimized substrate/product inhibition.
• Solving problems with expertise and creativity, as well as providing complete and professional interpretation of data to understand experimental results, and better integrate external research with internal data.
• Fully considering the specific applications and limitations of rational design, directed evolution and semi-rational design, selecting appropriate strategies to optimize engineered enzymes for different industrial applications, and promote green industrial development.

Creative BioMart has created successful high-quality enzymes for the industry solutions for our customers. We have professionals who listen carefully to your needs and can provide you with comprehensive support before, during and after your experiment. Each project will be individually designed to meet the needs of our customers and described in a detailed project report. Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our solutions, please contact us without hesitation.

Reference

1. Victorino da Silva Amatto I, Gonsales da Rosa‐Garzon N, Antônio de Oliveira Simões F, et al.. (2021) Enzyme engineering and its industrial applications[J]. Biotechnology and Applied Biochemistry.

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