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DEVELOPING PROTEIN ENGINEERING
Making any protein possible

Rooted in protein rational design and directed evolution guided engineering, our platform makes any protein possible. Creative BioMart provides the most comprehensive customized protein engineering related services and solutions for the biotechnology industry.

Protein engineering is an emerging biotechnology. On the basis of genetic recombination technology, biochemistry, molecular biology, molecular genetics and other disciplines, it integrates protein crystallography, protein dynamics, protein chemistry, and computer-aided design technologies.

Its content mainly has two aspects: (1) Synthesizing protein with specific amino acid sequence and spatial structure according to needs. Determine the relationship between protein chemical composition, spatial structure and biological function. (2) Realizing the prediction of the spatial structure and biological function of the protein from the amino acid sequence, and design and synthesize brand-new proteins with specific biological functions.

The fascinating field of protein engineering has provided breakthroughs by producing plethora of specifically engineered/rationally designed proteins with different functionalities and wide-scale applications in industrial, biotechnological, and pharmaceutical sectors. Mainly including improvements involving food, detergent, textile industry and the activity/function of enzymes that control environmental pollution. Protein-based treatment methods, vaccines and stents have greater safety, higher effectiveness, lower immunogenicity and better delivery function. Nanomaterials with enzyme-like activity are delivered in cells.

At Creative BioMart, everything starts and ends with protein engineering platform in an iterative process of insight and innovation.
Protein engineering strategy

Fig 1. Current dominant approaches for protein engineering. (Zhang G, et al., 2020)

Our protein engineering is based on directed evolution and rational design strategies. Screen or select from designing proteins, mutagenizing proteins, and evaluating improved properties of protein variants. We can design and modify the gene encoding protein purposefully according to your needs, and obtain a genetically modified biological system that can express protein through gene recombination technology.

Protein modification

Fig 2. Post-translational modification of proteins using chemical biology tools. (Anne C. Conibear, 2020)

The diversity of protein functions is also produced by post-translational modifications. Through post-translational modification, the type of protein can be increased explosively, and the structure/function relationship of the protein is changed, so that the function of the protein is more perfect and the regulation is more refined. We can provide services based on chemical modification and molecular biology modification according to your requirements.

Evolving smarter and faster to maximize the potential of protein engineering
Welcome to discover our advantages
Efficient and large-capacity screening

We use site saturation mutagenesis at many selected locations to design the library according to the characteristics to be improved. Through our proprietary machine learning software tools, we can effectively select in this complex and large-scale screening, reducing the need for subsequent screening.

Comprehensive design

Our platform combines directed evolution with rational computer design, and uses massively parallel sequencing to explore the sequence functional landscape of various proteins in depth. Deep sequencing and machine learning allow us to cover more sequence space, thereby increasing the possibility of identifying variants with specific and improved performance characteristics.

Professional team

Creative BioMart's team of computational biologists has extensive experience in protein engineering data analysis, statistics, statistical learning methods, and molecular dynamics and simulation. We use the public domain and proprietary custom tools to build a stable and rigorous framework for rapid development and testing. We can unravel the underlying molecular mechanism of amino acids and use computer data to predict the spatial structure and function of proteins.

Interested in cooperating with our research? Contact us today.

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References:

  1. Zhang G, Chen Y, Du G. 2020. Protein engineering approaches for enhanced catalytic efficiency. Biomass, Biofuels, Biochemicals. 103-117.
  2. Conibear, A.C. 2020. Deciphering protein post-translational modifications using chemical biology tools. Nat Rev Chem. 4, 674–695.