Creative BioMart provides a variety of services based on protein directed evolution strategies, including the construction and screening of mutation libraries, gene amplification, successful protein engineering from utilizing directed evolution, and so on. With years of experience in protein engineering, scientists of Creative BioMart provide you with professional and economic directed evolution service projects. Each project will be individually designed to meet the needs of our customers and described in a detailed project report. Our customers have direct access to our experts and we give timely feedback to any online inquiries. If you are interested in our services, please contact us without hesitation.

Directed Evolution

Traditional protein engineering methods (reasonable design) cannot meet the requirements for molecular improvement of existing proteins. Faced with this situation, directed evolution has gradually received attention. Directed evolution is to simulate the natural evolution process in the laboratory, using error-prone PCR, DNA shuffling, random guided recombination, staggered extension and other techniques for mutagenesis and recombination of in vitro mutant genes, designing high-throughput screening methods to select the required mutant strains.

Fig 1. Schematic overview of the principal processes, strategies, and techniques of directed evolution. (Jäckel C, et al., 2008)

Custom Services

Compared with rational design, directed evolution can obtain new proteins with expected characteristics when the structure of the protein is not clear. Directed evolution of protein is an effective method of protein engineering, which is of great significance for studying the relationship between protein structure and function. The field of its application is gradually expanding, covering all aspects of industry, agriculture, medicine, food and so on. Our protein directed evolution services include but not limited to:

• Protein Mutation Library Construction Services
• Protein High-Throughput Screening Services
• Gene Amplification Services
• Successful Protein Engineering from Utilizing Directed Evolution

Competitive Advantages

• Flexible design: customize and design various mutation libraries according to your needs.
• Efficient synthesis ability: rich experience in gene synthesis and library construction ensures the diversity and capacity of the library.
• Reliable gene sequencing and analysis services: match the appropriate sequencing plan according to your research purpose.
• Reduced workload: compared with traditional mutagenesis methods, the screening effort is significantly reduced. It is possible to generate variants that cannot be created using traditional methods.
• Maximum service: maximum sequence integrity and maximum variation in the unmutated region, which improves the possibility of obtaining useful variants.

Reference

1. Jäckel C, Kast P, et al.. (2008) Protein design by directed evolution. Annu. Rev. Biophys., 37, 153-173.

• Protein Engineering Rational Design Services
• Protein Modification Services
• Protein Synthesis Services