Creative BioMart is a well-known expert who uses various protein domains as non-antibody scaffolds to produce synthetic binding proteins that have the ability to bind to molecules. With years of experience, we successfully designed β-Barrel binding protein based on non-antibody scaffolds to precisely meet customer requirements.

Introduction of β-Barrel Protein

Outer membrane proteins are found in Gram-negative bacteria, mitochondria and chloroplasts. They form β barrels, so they are also called β barrel membrane proteins (βMPs). βMPs have a wide range of different functions, they can be non-specific diffusion pores (OmpA, OmpC, OmpF), specific pores (LamB, ScrY), active transporters (FhuA, FepA, BtuB), proteases (OmpT) , Lipase (OmPlA), Acyltransferase (PagP), etc. These βMPs play a key role in nutrient acquisition, protein input/output, and other basic biological processes. In recent years, the folding process, crystallization, observed limited structural diversity and the manifold channel engineering options of these β-barrel proteins have attracted widespread attention. In addition, more new β-barrels have been produced in protein design and protein engineering for disease treatment.

Fig 1. The evolutionary history of beta-barrels. (Nanda V, 2019)

Services

βMPs play an important role in bio-nanotechnology and druggability. They are involved in processes such as outer membrane biogenesis, membrane anchoring, pore formation, translocation of virulence factors, and enzyme activity. The existing protein forms have been adjusted through protein evolution to build larger β-barrels. As a leading service provider of protein engineering, Creative BioMart can accurately predict the 3D structure of βMPs, as well as accurately predict the structure of βMPs with a limited number of sequences and βMPs with new folds. In addition, we can also design new β-barrel proteins for disease treatment. Our βMPs engineered protein nanopores can spontaneously fold into the expected structure and be embedded in the lipid membrane, which can be used to sequence the genetic material of epidemics and discover mutant strains.

Creative BioMart has established a mature βMPs design platform. For example, the βMPs engineered protein nanopores we designed can spontaneously fold into the expected structure and be embedded in lipid membranes for sequencing the genetic material of epidemics and discovering mutant strains. The novel β-barrel protein incorporates a synthetic metal catalyst into the novel β-barrel protein scaffold to produce a biological hybrid catalyst. β barrel membrane protein for genome identification and screening. The βMPs design platform is widely used in the basic research of membrane protein folding and design, ion conductance and gating mechanism, and has many advantages and prospects in more application fields of random single-molecule sensing of proteins and proteins.

Based on the protein engineering platform, we have successfully designed a variety of platforms for different applications to produce special proteins. We will work with you to develop the most appropriate strategy and provide the most meaningful data for your research for accelerating the research of life sciences. If you are interested in our services, please do not hesitate to contact us for more information.

References

1. Nanda V. (2019) Building bigger beta-barrels. Elife. 8: e44076.
2. Schulz GE. (2000) beta-Barrel membrane proteins. Curr Opin Struct Biol. 10(4): 443-447.

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