Creative BioMart provides E. coli bacteria as a host display system service to screen out target proteins or peptide fragments from a huge random library to obtain highly active mutant enzymes or improve the immunity of antigenic determinants. With years of protein engineering research experience, we can provide you with the best quality and most professional services. If you are interested in our services, please do not hesitate to contact us for more information.

Escherichia coli is currently the most commonly used host for protein engineering through bacterial surface display, mainly because it has the ability to produce recombinant proteins and is easy to genetic manipulation. As a gram-negative bacteria, E. coli has an inner membrane and an outer membrane separated by a periplasmic space. In this system, membrane proteins native to E. coli are used as convenient targeting and anchoring parts, but heterologous anchoring proteins are also used.

A significant advantage of E. coli surface display platforms is that relatively high transformation efficiencies of E. coli allow libraries of 10⁹–10¹¹ members to be easily accessible. Creative BioMart has provided various E. coli surface display systems that mainly promote the surface exposure of peptides and small proteins:

Systems based on outer membrane proteins (OMPs) services

Different protein scaffolds have been used as carriers. β-Barrel proteins are integral membrane proteins, which span the OM with antiparallel β-sheets that form a barrel-like structure. We provide based on E. coli b-barrel outer membrane proteins (OMPs), such as LamB, FhuA, and the porins OmpA, OmpC and OmpX. These systems allow larger carrier proteins to be inserted on the surface of bacteria.

Systems based on surface appendages services

Flagella, pili or fimbriae are attachments on the surface of bacteria. We provide E. coli-based flagella, pili or fimbriae as carrier proteins, allowing small peptides to be inserted into exposed sites and displaying heterologous peptides inserted into exposed sites.

Systems based on ice nucleation protein (INP) services

INP is a secreted protein found in bacteria such as pseudomonas syringae, which can make host cells form ice crystal nuclei. The unique structure and function of INP make it very suitable for bacterial surface display system. We provide E. coli-based IPN as carrier proteins, allowing IPN to be expressed at a high level on the cell surface of E. coli without affecting the viability of the cell.

Systems based on virulence factors services

Several pathogenic bacteria express highly specific virulence factors on their cell surfaces, and these factors are usually necessary conditions for the occurrence and development of diseases. We provide E. coli-based invasion protein and the intimin protein as carrier proteins. Due to their unique modular structure, these bacterial virulence factors are good substitutes for β-barrel OMP-based peptide and small protein display systems.

Fig 1. Cell organelle associated surface display mechanism in Gram negative bacteria. (Shabnam Parwin, et al., 2011 )

References

1. Van Bloois E, Winter RT, et al.. (2011) Decorating microbes: surface display of proteins on Escherichia coli. Trends Biotechnol. 29(2):79-86.
2. Sharadwata Pan, Michael K. Danquah. (2011) Cell Surface Display[D]. Biodiversity, 63-74. Doi: 10.5772/23070.

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