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As an industry-leading monoclonal antibody production service provider, Creative BioMart has rich experience and unique insights in monoclonal antibody production, and can provide you with various customized monoclonal antibody production services to accelerate your research.
Monoclonal antibodies are highly homologous antibodies produced from a single B-cell clone against a specific epitope only. B cells with the ability to secrete specific antibodies and myeloma cells with unlimited growth ability are usually fused into B cell hybridomas by hybridoma technology. Taking advantage of the characteristics of such hybridoma cells, highly homogeneous antibodies directed against the same antigenic determinant are produced.
Fig 1. Process for the production of monoclonal antibodies by the hybridoma. (Michnick, S. W., Sidhu, S. S, 2013)
Creative BioMart relies on professional laboratory facilities to provide you with customizable, comprehensive and professional monoclonal antibody services to meet your various needs. Creative BioMart's monoclonal antibody production service includes but is not limited to the following items.
Mouse monoclonal antibodies can target a single antigenic determinant, with high specificity, stable passage, and large-scale reproducible production. They are widely used in basic research, medical diagnosis, tumor targeted therapy, radioimmunography and other fields.
Compared with mouse monoclonal antibodies, rabbit monoclonal antibodies have higher affinity and specificity, can recognize more epitopes, and can recognize mouse antigens.
Phosphorylation antibodies can specifically recognize specific phosphorylation sites and detect the increase or decrease of the phosphorylation level of the protein when cells are stimulated, thereby reflecting the changes in the functional state of the protein.
Each B cell contains only a functional pair of heavy and light chains, producing only one characteristic of a specific antibody. Therefore, monoclonal antibodies can be obtained by directly amplifying antibody genes from single B cells.
This is an antibody that can specifically recognize and bind to the idiotype of another antibody. It is widely used in the field of drug research and development, mainly in the immunogenicity analysis and pharmacokinetic analysis of antibody drugs.
Antigens that can stimulate the body to produce a strong immune response are the basis for the successful preparation of monoclonal antibodies. We promise to provide you with the highest quality antigen design services to lay the foundation for monoclonal antibody production.
The higher the purity of the antigen used to prepare the monoclonal antibody, the higher the success rate of the monoclonal antibody preparation experiment. In general, antigens can be proteins (natural or recombinant), polypeptides, small molecules, and the like.
According to a pre-established immunization scheme, animals are immunized with the prepared antigen, and corresponding B lymphocyte clones are stimulated to activate, proliferate, and differentiate into sensitized B lymphocytes.
Under the action of polyethylene glycol, various lymphocytes of the immunized animal fuse with myeloma cells to form hybridoma cells.
The positive wells after antibody determination can be expanded and cloned to obtain the progeny of single cells to secrete monoclonal antibodies. There are many methods of cloning, such as the limiting dilution method, soft agar method, single-cell micromanipulation, micromanipulation of monoclonal cell populations, and fluorescence-activated cell sorter (FACS) separation.
The cloned hybridoma cells are used to produce the target monoclonal antibody, and the monoclonal antibody is purified by methods such as affinity chromatography.
If you have any interest in our monoclonal antibody production service, please feel free to contact us. We look forward to a detailed discussion with you, leading to project collaboration.
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