In recombinant protein research, selecting the most suitable protein expression system and designing a suitable plasmid backbone, protein tag, and localization signal are very important. The expression of recombinant proteins first requires the design of protein expression constructs, which requires us to analyze the DNA sequence characteristics of the target protein and understand the protein's solubility, crystallinity, and functional properties. Then it is cloned into a suitable recombinant expression vector and cloned to express the target product.

Creative BioMart's strong expertise in protein expression construct design enables us to accelerate your project by providing the highest quality protein expression construct design service. This service includes the selection of expression systems, the design of expression structures, cloning and expression, etc., which can help researchers who study recombinant proteins in the fields of medicine and drug development.

Fig 1. Design of fluorescently labeled Cre-inducible miRNA-expression construct. (Qiu, L, et al., 2008)

Expression Construct Design Service

Creative BioMart can provide the most cost-effective protein expression construct design service with the fastest turnaround time to meet your research needs. We offer the following projects for your scientific research.

• Choice of Expression System

We will choose different expression systems according to the different translation modification requirements and characteristics of your target protein, such as the E. coli expression system, insect cell expression system, and mammalian cell expression system. Different expression systems have their advantages in growth rate, expression level, and post-translational modification.

• Design of Expression Construct

According to the information we obtained, such as the domain type of the target protein, protein solubility, stability, etc. And whether the protein contains a large number of disulfide bonds, whether it contains post-translational modifications, etc., to design the construct. At the same time, we also use bioinformatics tools to predict protein structures to guide the design of constructs.

We also need to determine the type of protein tag that needs to be included in the construct, usually using an affinity tag that facilitates purification/detection. In the later protein purification process, the purpose of removing the affinity tag or the solubility enhancing tag can be achieved by including a specific protease cleavage site between the tag and the target protein. In addition, the inserted promoter sequences, restriction sites, operons, origins of replication, and screening phenotypic tags need to be determined according to your purpose.

• Clonal Expression

Finally, we provide appropriate cloning and expression methods according to the characteristics of the expression vector. We will choose the method in the design that best fits the target protein.

Please feel free to contact us if you have any questions about our expression construction design service. We look forward to working with you on your research projects.

Reference

1. Qiu, L., et al. (2008). A construct with fluorescent indicators for conditional expression of miRNA. BMC biotechnology, 8, 77.

• Cloning and Subcloning Service
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• Protein Concentration Service
• Protein Expression Services
• Protein Purification Service
• Protein Refolding Service