Supported by our established advanced protein engineering platform, Creative BioMart, with its strong expertise in protein expression, can provide you with a comprehensive and customized service for the expression of proteins in yeast.

The yeast expression system is one of the most efficient eukaryotic protein expression systems, which can successfully achieve intracellular expression or secretory expression. Its amplification medium is relatively cheap, with low requirements for culture conditions, and is suitable for industrial amplification. Yeast contains a unique and powerful alcohol oxidase gene promoter, and the expression of exogenous genes can be strictly regulated by methanol. The exogenous protein gene of yeast is genetically stable, and the exogenous gene can be integrated into the yeast genome with a high copy number, thereby improving the expression strain. Yeast has the subcellular structure of eukaryotes and has post-translational modification processing functions such as glycosylation, fatty acylation, and protein phosphorylation. Commonly used yeast expression systems include Saccharomyces cerevisiae expression system, methanol yeast expression system, etc. Among them, the P. pastoris expression system has the advantages of a fast growth rate, abundant commercial expression vectors, and efficient secretion expression. It has become the preferred choice for yeast to express recombinant proteins and is widely used in the production of recombinant proteins, especially eukaryotic proteins.

Fig 1. The flowchart of recombinant protein expression in yeast. (Wang, G, et al., 2017)

Service Content of Protein Expression in Yeast

• Construction of Recombinant Yeast Expression Plasmid. We amplify, purify, and clone the target gene into a suitable expression plasmid by PCR. Finally, the accuracy of the constructed plasmid was verified by sequencing.
• Electro Transformation of Recombinant Plasmids. Subsequently, we electroporated the constructed expression plasmid into a high-efficiency yeast expression strain, and at the same time used protease to remove the unnecessary purification tag, so as to facilitate subsequent purification of the desired target protein.
• Recombinant Expression Screening. Positive clones were expanded in the culture medium, and methanol was added to induce the expression of the target protein. Then use SDS-PAGE electrophoresis to detect the expression of the target protein, and select a suitable monoclonal strain for the expression of the target protein.
• Protein Expression and Purification. We scale up the recombinant yeast by shaking flasks and inducing it to express the target protein. The expressed recombinant protein is then purified by various chromatographic methods such as affinity chromatography, ion exchange, hydrophobicity, and gel filtration. Finally, the purified protein was detected by SDS-PAGE electrophoresis, and the target protein was confirmed by WB.

Deliverables

• Sequencing report
• Purified protein
• Quality inspection report
• Experimental report

Our Advantages

• Faster delivery of experimental results and more cost-effective experimental quotations.
• Design different personalized experimental protocols according to protein characteristics or customer needs.
• With a high-throughput screening platform, the optimal expression conditions can be obtained quickly and efficiently.
• With a complete yeast expression platform and rich expression experience, it is guaranteed to prepare the protein that meets your ideal for you.

If you have any requests for our protein expression services in yeast, please feel free to contact us. We look forward to providing you with attractive projects.

Reference

1. Wang, G., et al. (2017). Exploring the potential of Saccharomyces cerevisiae for biopharmaceutical protein production. Current opinion in biotechnology, 48, 77-84.

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