With the support of our advanced protein engineering platform that we have established for many years, Creative BioMart has successfully designed a variety of platforms for different applications to produce special proteins based on the directed evolution strategy. Creative BioMart provides customers with a wide range of engineered protein-based biomaterials services, which exploit the advantages of synthetic and natural polymers to design novel artificial proteins with precisely controlled physical, chemical and biological properties that mimic a wide variety of natural biological materials.

Protein-engineered biomaterials are designed and synthesized using recombinant protein technology, creating an attractive alternative to synthetic polymeric scaffolds or harvested natural scaffolds. Protein-engineered biomaterials consist of modular peptide domains with various functions. These domains are encoded into DNA plasmids, transfected into selected organisms, and expressed and purified to produce biopolymers with precise molecular-level sequence specifications. Due to the modular design strategy of protein-engineered biomaterials, these scaffolds can be easily modified to optimize specific tissue engineering applications. Furthermore, this approach allows the direct encoding of multiple functional domains into the protein backbone, resulting in a vast array of biomaterials with tunable mechanical, biochemical, and biodegradable properties.

Fig 1. Schematic representation of selected modular protein-engineered biomaterials. (Mulyasasmita W, et al., 2011)

Services

The modularity, tunability, and sequence specificity of protein-engineered biomaterials make them attractive candidates for use as substrates for various tissue engineering applications. Creative BioMart has successfully designed a variety of protein biomaterials as multifunctional platforms to study and solve various challenges in regenerative medicine. Our engineered protein and peptide biomaterials are widely used to improve the effectiveness and delivery of subunit vaccines for infectious diseases, cancer, and autoimmune diseases. They are biodegradable and allow control over material structure and immune function. Our typical engineered biomaterials include, but not limited to:

• Protein Engineered Elastin Materials
• Protein Engineered Silk Materials
• Protein Engineered Coiled-coil Materials
• Protein Engineered Calmodulin Materials
• Protein Engineered Enzymatically-crosslinkable Materials
• Protein Engineered Collagen Materials

Strategies of Design Protein Biomaterials

Traditional biomaterials such as synthetic polymers are often biologically inert and/or may degrade into toxic fragments. The properties of natural polymers are not easily controlled and sources are limited. Combining the desirable properties (biocompatibility and tunability) of natural and synthetic biomaterials, our scientists utilize multiple design strategies to develop biomaterials to mimic multiple properties of natural extracellular matrix, including cell adhesion, cell signaling, elasticity and biodegradability. For your project, we will choose the right strategy for you.

• Peptide sequences obtained from wild-type proteins: we combined engineered proteolysis-sensitive modules with wild-type modules to yield a series of protein biomaterials with the same cell-binding properties, the same initial mechanical strength, and tunable degradation rates.
• Computationally guided amino acid sequence: an amino acid sequence is predicted by computational design, the sequence is folded into a WW-domain, and combined with peptide modules rich in other amino acids. Higher affinity compared to wild-type WW-domain.
• Peptide sequences discovered by high-throughput screening: we select appropriate codon sequences for specific expression systems to optimize protein yield, including E. coli display systems, yeast display systems, and phage display systems.

Service Principle

Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.

We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. Please do not hesitate to contact us for more information or to discuss in detail, if you are interested in our services.

References

1. Mulyasasmita W, Heilshorn S C. (2011) Protein-Engineered Biomaterials: Synthesis and Characterization.
2. Sengupta D, Heilshorn S C. (2010) Protein-engineered biomaterials: highly tunable tissue engineering scaffolds. Tissue Engineering Part B: Reviews.16(3): 285-293.

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