Creative BioMart's strong expertise in protein expression allows us to accelerate your projects by offering professional protein expression services. As a leading protein expression service provider, Creative BioMart has rich experience in protein expression and can customize various proteins for you. We can not only complete the preparation of small amounts of protein but also construct high-expression cell lines to meet your large-scale protein needs.

There are various protein expression systems, including bacterial expression systems dominated by Escherichia coli, yeast expression systems dominated by Pichia methanolica, insect cell expression systems, and mammalian cell expression systems. The protein products obtained by different expression systems are different, which are mainly reflected in the expression amount, the difficulty of separation and purification, the degree of translation processing, and the biological activity. At the same time, the price, cost, and experimental period are also different. We will choose the appropriate expression system according to your actual experimental needs.

Fig 1. The flowchart of protein expression.

Protein Expression Services

Creative BioMart can provide the most professional, shortest service cycle, and most competitively priced protein expression services to meet your various needs. We offer the following protein expression services for your research.

• Protein Expression in Bacterial Cells

Escherichia coli-based protein expression system is currently the most widely used and most economical bacterial protein expression system. It has many advantages, such as clear genetic background, fast cell proliferation, high expression, good stability, and strong anti-pollution ability.

• Protein Expression in Yeast

This is an exogenous protein expression system, which takes into account the advantages of the eukaryotic expression system and prokaryotic expression system, can carry out high-level protein expression, and can modify the expressed protein. At the same time, the medium price is affordable and the cultural conditions are not high, so it is suitable for industrial scale-up.

• Protein Expression in Insect Cells

The expression vector constructed by using the strong promoter of the polyhedrin protein in the baculovirus structural gene can make many eukaryotic target genes express efficiently or even at a high level. It can correctly post-translationally modify the protein so that the recombinant protein is closer to the natural protein in structure and function.

• Protein Expression in Mammalian Cells

The mammalian expression system has many advantages in protein initiation signal, processing, secretion, and glycosylation. The activity of exogenous protein produced by post-translational processing and modification far exceeds that of other expression systems, and is suitable for expressing complete macromolecular proteins.

If you have any special requirements for our protein expression services, please feel free to contact us. We look forward to working with you on attractive projects.

• Cloning and Subcloning Service
• Expression Construct Design Service
• Monoclonal Antibody Production Service
• Protein Concentration Service
• Protein Purification Service
• Protein Refolding Service