With the support of our advanced protein engineering platform established for many years, Creative BioMart has developed multiple cell-free surface display platforms, including ribosome display, mRNA display, covalent and non-covalent DNA display, and in vitro compartmentalization. Our scientists provide you with a comprehensive customized cell-free surface display system service for protein engineering.

The cell-free surface display system is also called the in vitro translation system, which reproduces the transcription and translation process of intracellular proteins by simulating the life phenomenon of biological cells. The cell-free protein display system is based on the ribosomal protein synthesis system and consists of cell extracts from E. coli, wheat germ or rabbit reticulocytes. It contains components required for protein production, such as ribosomes, translation factors, aminoacyl-tRNA synthetase and tRNA. In vivo gene display systems require complex transformation steps to prepare proteins, while cell-free protein display systems can directly use PCR products as templates to synthesize proteins. The cell-free protein display system has attracted much attention due to its simplicity and high controllability. It has shown good application prospects in terms of in vitro recombinant protein production, high-throughput protein synthesis, functional proteomics research, and in vitro directed evolution of proteins.

Fig 1. A system for protein evolution based on cell-free translation. (Shimizu Y, et al., 2006 )

Advantages of Cell-Free Surface Display System

The cell-free display system represents an alternative method of in vivo expression, greatly improving protein production efficiency. It has the following advantages:

• The reaction system is small and can synthesize many different proteins in parallel at the same time.
• The reaction cycle is short, which can meet the scientific research requirements of high-throughput ligand screening and proteomics.
• The open reaction system facilitates the change of various reaction conditions, is conducive to regulating gene transcription, protein synthesis and post-translational modification, and avoids the formation of inclusion bodies.
• The stable reaction system can be coupled with other processes to form an automated, programmed, and large-scale production, speeding up the purification, functional characterization and subsequent structural analysis of recombinant proteins.
• Without cell structure restrictions, it can be used to produce foreign proteins that are toxic to the host and avoid the lethal effect of protein expression on host cells.
• Unnatural amino acids or isotope-labeled amino acids can be added to express special proteins.

Cell-Free Surface Display System

There are many ways to link genotype and phenotype in a cell-free translation system. The further development of its subsystems, as well as the improvement of the cell-free translation system itself, will make these technologies more widely used. Our scientists have established a mature cell-free display system for you to choose from:

• Ribosome Displays Service.
• mRNA Displays Service.
• Covalent DNA Displays Service.
• Noncovalent DNA Displays Service.
• In Vitro Compartmentalization Displays Service.

Applications of Cell-Free Surface Display System

• Enhancing the synthesis of biologically active proteins.
• Creation of cell-like compartments.
• Synthetic artificial protein.
• Peptide evolution.
• Protein high-throughput screening.

Service Principle

Creative BioMart is based on innovative, pragmatic and honest, adhering to the tenet of "Quality is our life, providing customers with the best quality service", providing customized services to customers around the world.

We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. Please do not hesitate to contact us for more information or to discuss in detail, if you are interested in our services.

References

1. Shimizu Y, Kuruma Y, et al.. (2006) Cell-free translation systems for protein engineering. FEBS J. 273(18): 4133-40.
2. Contreras-Llano LE, Tan C. (2018) High-throughput screening of biomolecules using cell-free gene expression systems. Synth Biol (Oxf). 3(1): ysy012.

• Cell Surface Displays Service
• Phage Displays Service