Creative BioMart is a well-known expert specializing in the application of advanced phage display technology to accelerate the release of protein engineering. With years of experience, we are able to provide single-stranded filamentous phage (M13) display system service based on phage display technology to precisely meet customer requirements.

M13 phage has strong replication ability in filamentous phage, can hold a large amount of foreign DNA, and is the most commonly used phage display vector. M13 is an E. coli-specific filamentous bacteriophage with a genome of 6.4 kb, consisting of a circular single-stranded DNA (ssDNA) genome. It encodes 5 structural proteins, including the major capsid protein PVⅢ and the minor capsid PⅢ, PVΙ, PVⅡ and PΙX. Among them, PⅢ and PVⅢ are the two most commonly used proteins in phage display. Based on the M13 phage display technology, Creative BioMart provides the following system services:

PⅢ display system services

PⅢ is a secondary peripheral protein of the virus, located at one end of the virus particle. Each virus particle has 3 to 5 copies of PⅢ protein, which can be inserted into the N-terminal flexible junction region with foreign proteins or polypeptides.

The advantage of the PⅢ system we provide is that there is no strict requirement on the size of the foreign protein displayed, and it can be used to display proteins with larger molecular weights.

The main purpose of the PⅢ system we provide is to prepare phage antibodies, and its outstanding advantage is that it mimics the natural immune selection system.

PVⅢ display system services

PVⅢ is the main capsid protein of filamentous phage, which is distributed on both sides of the phage particle. Each virus particle has 3000 copies of PVⅢ protein.

The advantage of the PⅢ system we provide is that it contains 32 amino acid residues, its C-terminus is combined with phage DNA to form the inner wall of the phage envelope, which can accommodate smaller foreign fragments.

The PVⅢ system we provide is more suitable for screening low-affinity ligands due to the large number of copies of the PVⅢ protein.

Fig 1. A typical representation of M13 phage with about 1,000 nm in length and 5 nm wide. (Saw PE, et al., 2019 )

Features of M13 Phage Display System

• The M13 phage shell is extremely malleable, which can be used to design phage particles specifically for phage display.
• The polypeptide fused with the M13 coat protein will be displayed on the surface of the phage particle, provided that the fusion protein can successfully pass through the assembly device and be integrated into the assembled phage without significantly affecting the viability of the phage particle itself.
• The multiple capsid proteins of M13 provide comprehensive display options, which can be used to display various peptides and proteins with unique characteristics.

Creative BioMart has been continuously developing cutting-edge phage display technology, and is committed to providing flexible and efficient customized M13 display system service through the PⅢ display system and PVⅢ display system. Based on the protein engineering platform, we have successfully constructed an impeccable phage display library of various types. We are proud to be able to screen and select proteins with the required characteristics from a large number of variants to best accelerate the research of life sciences. If you are interested in our services, please do not hesitate to contact us for more information.

References

1. Saw PE, Song EW. (2019) Phage display screening of therapeutic peptide for cancer targeting and therapy. Protein Cell. 10(11): 787-807.
2. Bazan J, Całkosiński I, Gamian A. (2012) Phage display--a powerful technique for immunotherapy: 2. Vaccine delivery. Hum Vaccin Immunother. 8(12): 1829-35.
3. Levisson M, Spruijt RB, et al.. (2014) Phage display of engineered binding proteins. Methods Mol Biol. 1129: 211-29.

• T4 Phage Displays Service
• T7 Phage Displays Service
• λ Phage Displays Service