Creative BioMart provides a variety of services based on protein rational design strategies, including the computer graphics, homology modeling, and bioinformatics, and so on. With years of experience in protein engineering, scientists of Creative BioMart provide you with professional and economic computational design service projects. Each project will be individually designed to meet the needs of our customers and described in a detailed project report. Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our services, please contact us without hesitation.

Rational Design

Proteins generated by rational design can confer and control functions, improving the understanding of protein structure-function relationships. Approaches to rational design are based on a growing body of protein structures, biochemical data, robust models and computational methods. The rational design of proteins ranges from hypothesis-driven modification of proteins by inspection to advanced computer-aided design. In rational design, knowledge of a given protein can be obtained by X-ray crystallography, NMR, EM, etc. In addition, with the continuous improvement of computer computing power, the emergence of advanced algorithms, and the continuous mining and analysis of the relationship between protein sequence features, three-dimensional structures, and catalytic mechanisms, computer-aided protein design strategies have received unprecedented attention and development.

Fig 1. Schematic representation of rational design. (Prajapati A S, et al., 2018)

Custom Services

Compared with directed evolution, rational protein design can provide a clear transformation scheme, which greatly reduces the workload required for establishing and screening mutant libraries. At present, many achievements have been made in protein de novo design, substrate selectivity and thermostability design of enzymes. Rational design is a specific strategy of protein engineering that attempts to create improved protein molecules based on three-dimensional structure and the relationship between structure and function. Our protein rational design services include but not limited to:

• Protein Structure Prediction and Assessment Services
• Knowledge-Based Protein Design Services
• Rotamer Libraries for Molecular Modeling and Design of Proteins
• Computational Therapeutic Protein Design Services
• In-silico Pharmacology Services

In many studies, rational design is often used in conjunction with directed evolution to generate libraries containing high-frequency sequences with desired functional properties. Computational protein engineering overcomes some of the shortcomings of experimental approaches by exploring protein folds not observed in nature and designing new proteins from scratch (ie, de novo design). Our professional protein engineering team adopts the following rational design workflow to provide customers with a complete development cycle of rational design.

Competitive Advantages

• Rational design: relying on structural knowledge of the protein of interest to make the desired changes to the protein by directly manipulating its amino acids, reducing the workload.
• Rich knowledge: our protein engineering team is familiar with the theoretical basis of atomic physics, quantum physics, and quantum chemistry revealed by protein computational design, and the laws of microscopic particle motion, energy and interaction are based on statistical energy functions.
• Cutting-edge technology: with the aid of computers, through the use of a series of computational methods such as molecular docking, molecular dynamics simulation, quantum mechanical methods, Monte Carlo, and simulated annealing.
• One-stop service: we provide a complete set of services throughout the entire development cycle, providing you with single-destination testing and consulting services.

References

1. Prajapati A S, Panchal K J, Pawar V A, et al.. (2018) Review on cellulase and xylanase engineering for biofuel production[J]. Industrial Biotechnology. 14(1): 38-44.
2. Korendovych I V. (2018) Rational and semirational protein design[J]. Protein Engineering. 15-23.

• Protein Engineering Directed Evolution Services
• Protein Modification Services
• Protein Synthesis Services