Creative BioMart provides a variety of protein post-translational modifications to increase the functional diversity of the proteome, including chemical modification, site-specific modification, polyethylene glycol modification, molecular biology modification, etc. With years of experience in protein engineering, scientists of Creative BioMart provide you with professional and economic protein modification service projects. Each project will be individually designed to meet the needs of our customers and described in a detailed project report. Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our services, please contact us without hesitation.

Protein Modification

Proteins are fundamental functional units that perform cellular functions, and their expression is regulated by genome and epigenetics. Usually, the protein needs to be modified to different degrees after expression in order to perform the desired function. Post-translational modifications (PTMs) of proteins refer to chemical changes that occur after a protein is produced. It can affect protein structure, electrophilicity, and interactions, and studies have shown that there are more than 400 different types of PTMs that affect many aspects of protein function. PTMs increase the functional diversity of the proteome by covalently adding functional groups or proteins, regulating the proteolytic cleavage of subunits, or the degradation of whole proteins. These modifications include phosphorylation, glycosylation, ubiquitination, nitrosylation, methylation, acetylation, lipidation, and proteolysis, and are involved in important cellular processes including trafficking, differentiation, migration, and signaling play a key role. Therefore, replicating such natural modifications of proteins (by introducing native PTMs) in an efficient and controllable manner will provide an invaluable tool for studying their precise functions. Furthermore, identification and understanding of PTMs are critical in the study of cell biology and disease treatment and prevention.

Fig 1. Proteins in eukaryotic cells can be edited after translation by a wide variety of reversible and irreversible PTM mechanisms. (Wang Y C, et al., 2014)

Custom Services

Modifications of proteins are widespread in nature, widely increasing the diversity of protein structures. But experimental methods for identifying protein modifications are costly and difficult. Creative BioMart has successfully developed a number of computational methods for predicting post-translational modifications to add new functional groups to proteins:

(1) Enzymes can change protein structure by introducing new chemical groups to specific amino acids in the molecule.

(2) This can include phosphorylation, methylation, acetylation, glycosylation, ubiquitination, lipidation, biotinylation, etc.

(3) Adding chemical groups changes the properties of the chain and causes conformational changes, thereby affecting activity

(4) Modification of proteins by removing specific amino acid fragments from propeptides.

We use cutting-edge computer-based methods to identify novel modification sites and sequence-based computer programs to identify putative modification sites to provide high-quality protein modification services to global customers. We will choose the best protein modification method for you based on your needs. Our protein modification services include but not limited to:

• Protein Phosphorylation Service
• Protein Glycosylation Service
• Protein Nitrosylation Service
• Protein Methylation Service
• Protein Sulfation Service
• Protein Acetylation Service
• Protein Hydroxylation Service
• Protein Carbonylation Service
• Protein Ubiquitination Service
• Protein Proteolytic Service

Some traditional experimental methods are widely used in protein modification, including: immunoprecipitation, flow cytometry, two-dimensional gel electrophoresis, solid-phase metal affinity chromatography, based on liquid chromatography coupled with biological mass spectrometry (LC-MS) wait. Combined with experimental methods, a variety of computational methods are used to predict any specific type of PTM and perform PTM enrichment analysis on proteins. Our professional protein engineering team adopts the following post-translational modification prediction process to provide customers with a complete development cycle of proteins modifications.

Competitive Advantages

• Cutting-edge technology: calculation-based PTM prediction is very time-saving and labor-saving. A wealth of PTM data is available from various publicly accessible databases.

• Various types of protein modifications: based on adding chemical groups, based on adding complex groups, based on adding peptides, based on protein cleavage, based on amino acid modification, etc.

• Experienced team: our protein engineering team has solid expertise and years of research experience in post-biosynthetic amino acid side chain modification of proteins.

• One-stop service: we provide a full suite of services throughout the development cycle, providing you with single- destination testing consulting services.

References

1. Moore BR, Free SJ. (1985) Protein modification and its biological role. Int J Biochem. 17(3): 283-289.
2. Ramazi S, Zahiri J. (2021) Posttranslational modifications in proteins: resources, tools and prediction methods. Database (Oxford). 2021: baab012.
3. Wang Y C, Peterson S E, Loring J F. (2014) Protein post-translational modifications and regulation of pluripotency in human stem cells[J]. Cell research. 24(2): 143-160.

• Protein Engineering Directed Evolution Services
• Protein Engineering Rational Design Services
• Protein Synthesis Services