Creative BioMart's strong expertise in gene synthesis enables us to accelerate your project by providing protein mutation library construction services, including the construction of random and degenerate mutagenesis library, site-directed mutagenesis library, scanning mutagenesis library and homologous recombination library construction. This service can effectively provide thousands of protein variants, and can use in vitro molecular optimization methods to synthesize a reasonably designed or systematic mutation library into a single sequenced clone.

The protein mutation library contains many different protein variants and is becoming more and more popular. It can be used to identify key residues in protein structure and function and optimize protein function. These proteins exhibit enhanced expression levels, solubility, stability, enzymatic activity, or other differences. Need to combine the interaction of partners to better control physiological activities and pathological processes.

Fig 1. Construction of the mutant libraries. (Wu N C, et al., 2015)

Creative BioMart can provide the most affordable mutation library construction service with the fastest turnaround time to meet your downstream needs. Creative BioMart provides the following mutation library construction services for your scientific research:

• Random and Degenerate Mutagenesis Library Construction

With our in vitro library synthesis technology, random substitution can be introduced with maximum flexibility at a controllable level. The random mutation frequency can be precisely controlled in any form of 1.4 mutations/kb, 4-8 mutations/kb, and 8-12 mutations/kb.

With our degenerate oligonucleotide technology, any form of randomization of full-length genes can be generated in synthetic DNA fragments, allowing controlled, highly accurate randomization within oligonucleotides.

• Site-Directed Mutagenesis Library Construction

Prior to the development of site-directed mutagenesis, all mutations were random and scientists had to use selection of the desired phenotype to find the desired mutation. Site-directed mutagenesis is the introduction of selected changes into DNA in a precise and site-specific manner.

• Scanning Mutagenesis Library Construction

Scanning mutations uses alanine to replace the amino acids in each position or key position one by one, and finds the amino acid residues that have important functions for protein function, interaction and morphology. It is a systematic method to improve protein performance. Providing a detailed overview of each amino acid at that position.

• Homologous Recombination Library Construction

Homologous recombination is a method of introducing foreign genes into the chromosomes of recipient cells, where there is a sequence homologous to the introduced genes, and new gene fragments can replace defective gene fragments to achieve the purpose of correcting the defective gene.

If you have any special requirements about our construction services of protein mutation library, please feel free to contact us. We are looking forward to working together with your attractive projects.

Reference

1. Wu N C, Olson C A, Du Y, et al. (2015) Functional constraint profiling of a viral protein reveals discordance of evolutionary conservation and functionality[J]. PLoS genetics. 11(7): e1005310.

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