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In the expression and purification of proteins, the protein concentration will be reduced due to the liquid environment, and the next analysis and research of many proteins require high concentration or high purity samples. Therefore, increasing the protein concentration is very important for subsequent research. Creative BioMart is a leading protein engineering technology company that provides high-quality protein concentration services to our customers. Based on our years of experience and unique understanding of protein concentration, we are sure to provide protein concentration service to accelerate your project.
Protein concentration is mainly to remove water, impurity small molecules, etc. in the protein solution by physical or chemical methods, to increase the protein concentration in the target product. Protein concentration methods generally include adsorption, ultrafiltration, and precipitation. Different methods are different in protein molecular activity, protein stability, protein recovery rate, etc., and need to be selected according to the actual situation of the sample and the needs of the project.
Fig 1. The flowchart of the lupin protein concentration process. (Burgos-Díaz, C, et al., 2018)
Creative BioMart can provide the most affordable protein concentrate service with the fastest turnaround time to meet your downstream needs. Our service includes but is not limited to the following projects.
In this method, we can use the adsorbent to directly remove the water molecules in the protein solution, and the adsorbent in the reaction does not have any chemical reaction with the original solution, which is suitable for most proteins with poor stability. You can choose the dialysis bag concentration method or the gel concentration method according to your needs.
In this method, we use a special membrane that selectively filters various solute molecules in solution. Under certain pressure, the solvent and small molecules permeate, and the macromolecules are hindered and retained, thereby obtaining the target concentrated protein. This method is suitable for the concentration or desalting of macromolecular proteins.
The main principle of this method is to use the interaction of ionic groups of protein molecules in an aqueous solution, by changing the pH value or ionic strength of the liquid, and adding organic solvents or polymers to promote the precipitation of protein molecules. Finally, the concentrated protein is obtained by centrifugation, filtration, washing, and other steps. This method is suitable for concentration before further purification by chromatography.
In this method, we freeze the protein liquid at low temperature, remove water molecules by sublimation, and then vacuum dry it to obtain a protein-containing powder. This method can maximize the preservation of the natural components in the protein and is suitable for large-scale protein concentration.
If you have any questions about our protein concentration service, please feel free to contact us. We look forward to discussing the details with you and reaching attractive project cooperation.
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